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2018 ; 11
(ä): 23-38
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Cryostorage of immature and mature human testis tissue to preserve spermatogonial
stem cells (SSCs): a systematic review of current experiences toward clinical
applications
#MMPMID30013372
Zarandi NP
; Galdon G
; Kogan S
; Atala A
; Sadri-Ardekani H
Stem Cells Cloning
2018[]; 11
(ä): 23-38
PMID30013372
show ga
While the survival rate of children with cancer is increasing, preserving
fertility for prepubertal boys is still a challenge. Although intracytoplasmic
sperm injection (ICSI) using frozen sperms has revolutionized infertility
treatment, it is not applicable for the patients who undergo chemotherapy before
puberty since spermatogenesis has not begun. Therefore, preserving spermatogonial
stem cells (SSCs) as an experimental option can be provided to prepubertal
patients at a risk of damage or loss of their SSCs due to cancer treatments and
developmental or genetic disorders. Using frozen SSCs in testicular tissue,
successful SSC autotransplantation in mouse and nonhuman primates has shown a
promising future for SSC-based cell therapy. Cryopreservation of testicular
tissue containing SSCs is the first step to translate SSC-based cell therapy into
clinical male infertility treatment, and in the investigation into SSCs, it is
very important to evaluate their quantity and functionality during this process.
This systematic review summarizes the published data on cryopreservation
techniques in human testis tissue for potential utilization in future clinical
applications.