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2018 ; 11
(ä): 3805-3815
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Silencing of c-jun decreases cell migration, invasion, and EMT in radioresistant
human nasopharyngeal carcinoma cell line CNE-2R
#MMPMID30013361
Lin G
; Yu B
; Liang Z
; Li L
; Qu S
; Chen K
; Zhou L
; Lu Q
; Sun Y
; Zhu X
Onco Targets Ther
2018[]; 11
(ä): 3805-3815
PMID30013361
show ga
BACKGROUND: Previously, we found that c-jun was highly expressed in
radiation-resistant human nasopharyngeal carcinoma cells (CNE-2R) compared with
human nasopharyngeal carcinoma cells (CNE-2). MATERIALS AND METHODS: In this
study, we first used the scratch assays and transwell assays to detect the
migration and invasion of CNE-2R and CNE-2 cells and tested the epithelial
mesenchymal transformation (EMT)-related proteins E-cadherin and N-cadherin by
Western blot analysis. Subsequently, c-jun was knocked down to establish the
effect of c-jun on EMT, migration, and invasion of CNE-2R cells both in vitro and
in vivo. RESULTS: A high EMT level, CNE-2R cells were more capable of migration
and invasion than CNE-2 cells. Moreover, silencing of c-jun has upregulated the
expression of E-cadherin and downregulated N-cadherin in CNE-2R cells, and
subsequently the migration and invasion capacity of the cells was decreased.
Consistent with in vitro results, in vivo studies indicated that the c-jun
silencing reduced pulmonary migration of CNE-2R cells. Immunohistochemistry of
lung metastatic tumor tissue showed that E-cadherin was upregulated, and
N-cadherin was downregulated. CONCLUSION: These findings suggest that silencing
of c-jun in CNE-2R cells reduces cells migration, invasion, and EMT both in vitro
and in vivo.