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10.1371/journal.pone.0198823 http://scihub22266oqcxt.onion/10.1371/journal.pone.0198823 C6037349!6037349!29985912     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+One 2018 ; 13 (7): ä Nephropedia Template TP {{tp|p=29985912|t=2018. CTEN induces epithelial-mesenchymal transition (EMT) and metastasis in non small cell lung cancer cells |pdf=|usr=}}{{29985912}} gab.com Text CTEN induces epithelial-mesenchymal transition (EMT) and metastasis in non small cell lung cancer cells JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=29985912 #FOAvip To explore the effects and mechanism of CTEN (COOH-terminus tensin-like molecule) on EMT, cell migration and invasion of Human lung adenocarcinoma cells. The pCMV-vector, pCMV-CTEN, Control-shRNA, and CTEN-shRNA were transfected into A549 and NCI-H1299 cells by Lipofectamine 2000. Transforming growth factor-?1(TGF-?1)and epithelial-mesenchymal transition (EMT) -related biomarkers were detected by eliseand western blot. The migration and invasion ability of A549 cells and NCI-H1299 were examined by scratch-wound assay and transwell assay respectively. We found compare with control group, the expression of TGF-? and mesenchymal markers in CTEN overexpression group were increased, and the epithelial marker was decreased, which induced the EMT process. Meanwhile, scratch-woundassay showed that the migration efficiency of A549 and NCI-H1299 cells in CTEN overexpression group were higher than that in control group.Transwell assay demonstrated that the number of cells that migrated and invaded through the membrane were obviously more than those in control group.Furthermore, Knockdown of CTEN partially reversed transforming growth factor-?1(TGF-?1)-induced changes in EMT markers. In conclusion, CTEN activated the expression of TGF-?1, thereby prompting EMT in lung adenocareinma cancer cells. Twit Text FOAVip CTEN induces epithelial-mesenchymal transition (EMT) and metastasis in non small cell lung cancer cells ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=29985912 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29985912 #FOAvip English Wikipedia <ref>{{Cite pmid|29985912}}</ref> CTEN induces epithelial-mesenchymal transition (EMT) and metastasis in non small cell lung cancer cells #MMPMID29985912 Lu X; Gao J; Zhang Y; Zhao T; Cai H; Zhang T PLoS One 2018[]; 13 (7): ä PMID29985912show ga To explore the effects and mechanism of CTEN (COOH-terminus tensin-like molecule) on EMT, cell migration and invasion of Human lung adenocarcinoma cells. The pCMV-vector, pCMV-CTEN, Control-shRNA, and CTEN-shRNA were transfected into A549 and NCI-H1299 cells by Lipofectamine 2000. Transforming growth factor-?1(TGF-?1)and epithelial-mesenchymal transition (EMT) -related biomarkers were detected by eliseand western blot. The migration and invasion ability of A549 cells and NCI-H1299 were examined by scratch-wound assay and transwell assay respectively. We found compare with control group, the expression of TGF-? and mesenchymal markers in CTEN overexpression group were increased, and the epithelial marker was decreased, which induced the EMT process. Meanwhile, scratch-woundassay showed that the migration efficiency of A549 and NCI-H1299 cells in CTEN overexpression group were higher than that in control group.Transwell assay demonstrated that the number of cells that migrated and invaded through the membrane were obviously more than those in control group.Furthermore, Knockdown of CTEN partially reversed transforming growth factor-?1(TGF-?1)-induced changes in EMT markers. In conclusion, CTEN activated the expression of TGF-?1, thereby prompting EMT in lung adenocareinma cancer cells. äCTEN induces epithelial-mesenchymal transition (EMT) and metastasis in(epmc).pdf DeepDyve Pubget Overpricing