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10.1186/s12915-018-0545-0 http://scihub22266oqcxt.onion/10.1186/s12915-018-0545-0 C6033233!6033233!29976198     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       BMC+Biol 2018 ; 16 (ä): ä Nephropedia Template TP {{tp|p=29976198|t=2018. FlashFry: a fast and flexible tool for large-scale CRISPR target design |pdf=|usr=}}{{29976198}} gab.com Text FlashFry: a fast and flexible tool for large-scale CRISPR target design JO: BMC Biol http://www.kidney.de/pget.php?&tw=1&pmid=29976198 #FOAvip Background: Genome-wide knockout studies, noncoding deletion scans, and other large-scale studies require a simple and lightweight framework that can quickly discover and score thousands of candidate CRISPR guides targeting an arbitrary DNA sequence. While several CRISPR web applications exist, there is a need for a high-throughput tool to rapidly discover and process hundreds of thousands of CRISPR targets. Results: Here, we introduce FlashFry, a fast and flexible command-line tool for characterizing large numbers of CRISPR target sequences. With FlashFry, users can specify an unconstrained number of mismatches to putative off-targets, richly annotate discovered sites, and tag potential guides with commonly used on-target and off-target scoring metrics. FlashFry runs at speeds comparable to commonly used genome-wide sequence aligners, and output is provided as an easy-to-manipulate text file. Conclusions: FlashFry is a fast and convenient command-line tool to discover and score CRISPR targets within large DNA sequences. Twit Text FOAVip FlashFry: a fast and flexible tool for large-scale CRISPR target design ????BMC Biol http://www.kidney.de/pget.php?&tw=1&pmid=29976198 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29976198 #FOAvip English Wikipedia <ref>{{Cite pmid|29976198}}</ref> FlashFry: a fast and flexible tool for large-scale CRISPR target design #MMPMID29976198 McKenna A; Shendure J BMC Biol 2018[]; 16 (ä): ä PMID29976198show ga Background: Genome-wide knockout studies, noncoding deletion scans, and other large-scale studies require a simple and lightweight framework that can quickly discover and score thousands of candidate CRISPR guides targeting an arbitrary DNA sequence. While several CRISPR web applications exist, there is a need for a high-throughput tool to rapidly discover and process hundreds of thousands of CRISPR targets. Results: Here, we introduce FlashFry, a fast and flexible command-line tool for characterizing large numbers of CRISPR target sequences. With FlashFry, users can specify an unconstrained number of mismatches to putative off-targets, richly annotate discovered sites, and tag potential guides with commonly used on-target and off-target scoring metrics. FlashFry runs at speeds comparable to commonly used genome-wide sequence aligners, and output is provided as an easy-to-manipulate text file. Conclusions: FlashFry is a fast and convenient command-line tool to discover and score CRISPR targets within large DNA sequences. äFlashFry: a fast and flexible tool for large-scale CRISPR target desig(epmc).pdf DeepDyve Pubget Overpricing