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10.1038/s41598-018-28290-4 http://scihub22266oqcxt.onion/10.1038/s41598-018-28290-4 C6031611!6031611 !29973616     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=29973616 &cmd=llinks): Failed to open stream: HTTP request failed! 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Mycobacterium tuberculosis CarD, an essential global transcriptional regulator forms amyloid-like fibrils |pdf=|usr=}}{{29973616 }} gab.com Text Mycobacterium tuberculosis CarD, an essential global transcriptional regulator forms amyloid-like fibrils JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=29973616 #FOAvip CarD is an essential global transcription regulator from Mycobacterium tuberculosis (Mtb) that binds RNA polymerase and activates transcription by stabilizing the transcription initiation complex. Available crystal structures have captured two distinct, monomeric and domain-swapped homodimeric, oligomeric states of CarD. However, the actual oligomeric state of CarD in solution and its biological relevance has remained unclear. Here, we confirm the presence of the homodimeric state of CarD in solution by using synchrotron-based small-angle X-ray scattering. Furthermore, by using biochemical and biophysical experiments, in addition to mass-spectrometry, transmission electron microscopy, and confocal imaging, we show that CarD is the first soluble cytosolic protein in Mtb which displays the tendency to form amyloid-like fibrils both in vitro as well as in vivo. We demonstrate that the deletion of the fourteen N-terminal residues involved in domain-swapping hampers amyloid formation, thus, suggesting that domain-swapping is crucial in amyloidogenesis. The discovery of the amyloidogenic property of an essential cytosolic global transcription regulator, CarD, in a pathogenic bacteria will further open up new frontiers in research. Twit Text FOAVip Mycobacterium tuberculosis CarD, an essential global transcriptional regulator forms amyloid-like fibrils ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=29973616 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29973616 #FOAvip English Wikipedia <ref>{{Cite pmid|29973616 }}</ref> Mycobacterium tuberculosis CarD, an essential global transcriptional regulator forms amyloid-like fibrils #MMPMID29973616 Kaur G ; Kaundal S ; Kapoor S ; Grimes JM ; Huiskonen JT ; Thakur KG Sci Rep 2018[Jul]; 8 (1 ): 10124 PMID29973616 show ga CarD is an essential global transcription regulator from Mycobacterium tuberculosis (Mtb) that binds RNA polymerase and activates transcription by stabilizing the transcription initiation complex. Available crystal structures have captured two distinct, monomeric and domain-swapped homodimeric, oligomeric states of CarD. However, the actual oligomeric state of CarD in solution and its biological relevance has remained unclear. Here, we confirm the presence of the homodimeric state of CarD in solution by using synchrotron-based small-angle X-ray scattering. Furthermore, by using biochemical and biophysical experiments, in addition to mass-spectrometry, transmission electron microscopy, and confocal imaging, we show that CarD is the first soluble cytosolic protein in Mtb which displays the tendency to form amyloid-like fibrils both in vitro as well as in vivo. We demonstrate that the deletion of the fourteen N-terminal residues involved in domain-swapping hampers amyloid formation, thus, suggesting that domain-swapping is crucial in amyloidogenesis. The discovery of the amyloidogenic property of an essential cytosolic global transcription regulator, CarD, in a pathogenic bacteria will further open up new frontiers in research. |Amyloid/*chemistry/genetics [MESH] |Bacterial Proteins/*chemistry/genetics [MESH] |Mutation [MESH] |Mycobacterium tuberculosis/*metabolism [MESH] |Polymerization [MESH]Mycobacterium tuberculosis CarD, an essential global transcriptional r(epmc).pdf DeepDyve Pubget Overpricing