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Development of a screening strategy for new modulators of T cell receptor
signaling and T cell activation
#MMPMID29968737
Chen EW
; Brzostek J
; Gascoigne NRJ
; Rybakin V
Sci Rep
2018[Jul]; 8
(1
): 10046
PMID29968737
show ga
Activation of the T cell receptor (TCR) leads to the generation of a network of
signaling events critical to the developmental decision making and activation of
T cells. Various experimental approaches continue to identify new signaling
molecules, adaptor proteins, and other regulators of TCR signaling. We propose a
screening strategy for the identification of small molecules affecting TCR
signaling based on the uncoupling of TCR stimulation from cellular responses in
developing thymocytes. We demonstrate that this strategy successfully identifies
inhibitors of kinases already shown to act downstream of TCR engagement, as well
as new inhibitors. The proposed strategy is easily scalable for high throughput
screening and will contribute to the identification of new druggable targets in T
cell activation.
|Adaptor Proteins, Signal Transducing/metabolism
[MESH]
free
free
free
