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10.1038/s41598-018-28363-4 http://scihub22266oqcxt.onion/10.1038/s41598-018-28363-4 C6030044!6030044 !29968812     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29968812 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Sci+Rep 2018 ; 8 (1 ): 9987 Nephropedia Template TP {{tp|p=29968812 |t=2018. Lipid rafts are essential for release of phosphatidylserine-exposing extracellular vesicles from platelets |pdf=|usr=}}{{29968812 }} gab.com Text Lipid rafts are essential for release of phosphatidylserine-exposing extracellular vesicles from platelets JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=29968812 #FOAvip Platelets protect the vascular system during damage or inflammation, but platelet activation can result in pathological thrombosis. Activated platelets release a variety of extracellular vesicles (EVs). EVs shed from the plasma membrane often expose phosphatidylserine (PS). These EVs are pro-thrombotic and increased in number in many cardiovascular and metabolic diseases. The mechanisms by which PS-exposing EVs are shed from activated platelets are not well characterised. Cholesterol-rich lipid rafts provide a platform for coordinating signalling through receptors and Ca(2+) channels in platelets. We show that cholesterol depletion with methyl-?-cyclodextrin or sequestration with filipin prevented the Ca(2+)-triggered release of PS-exposing EVs. Although calpain activity was required for release of PS-exposing, calpain-dependent cleavage of talin was not affected by cholesterol depletion. P2Y(12) and TP?, receptors for ADP and thromboxane A(2), respectively, have been reported to be in platelet lipid rafts. However, the P2Y(12) antagonist, AR-C69931MX, or the cyclooxygenase inhibitor, aspirin, had no effect on A23187-induced release of PS-exposing EVs. Together, these data show that lipid rafts are required for release of PS-exposing EVs from platelets. Twit Text FOAVip Lipid rafts are essential for release of phosphatidylserine-exposing extracellular vesicles from platelets ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=29968812 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29968812 #FOAvip English Wikipedia <ref>{{Cite pmid|29968812 }}</ref> Lipid rafts are essential for release of phosphatidylserine-exposing extracellular vesicles from platelets #MMPMID29968812 Wei H ; Malcor JM ; Harper MT Sci Rep 2018[Jul]; 8 (1 ): 9987 PMID29968812 show ga Platelets protect the vascular system during damage or inflammation, but platelet activation can result in pathological thrombosis. Activated platelets release a variety of extracellular vesicles (EVs). EVs shed from the plasma membrane often expose phosphatidylserine (PS). These EVs are pro-thrombotic and increased in number in many cardiovascular and metabolic diseases. The mechanisms by which PS-exposing EVs are shed from activated platelets are not well characterised. Cholesterol-rich lipid rafts provide a platform for coordinating signalling through receptors and Ca(2+) channels in platelets. We show that cholesterol depletion with methyl-?-cyclodextrin or sequestration with filipin prevented the Ca(2+)-triggered release of PS-exposing EVs. Although calpain activity was required for release of PS-exposing, calpain-dependent cleavage of talin was not affected by cholesterol depletion. P2Y(12) and TP?, receptors for ADP and thromboxane A(2), respectively, have been reported to be in platelet lipid rafts. However, the P2Y(12) antagonist, AR-C69931MX, or the cyclooxygenase inhibitor, aspirin, had no effect on A23187-induced release of PS-exposing EVs. Together, these data show that lipid rafts are required for release of PS-exposing EVs from platelets. |Blood Coagulation/drug effects [MESH] |Blood Platelets/*metabolism/physiology [MESH] |Calcimycin/pharmacology [MESH] |Extracellular Vesicles/metabolism/physiology [MESH] |Filipin [MESH] |Healthy Volunteers [MESH] |Humans [MESH] |Membrane Microdomains/*metabolism/*physiology [MESH] |Membrane Proteins/metabolism [MESH] |Phosphatidylserines/metabolism [MESH] |Platelet Activation/drug effects [MESH] |Signal Transduction/drug effects [MESH] |Thrombin/metabolism [MESH]Lipid rafts are essential for release of phosphatidylserine-exposing (epmc).pdf DeepDyve Pubget Overpricing