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10.1038/s41467-018-04985-0 http://scihub22266oqcxt.onion/10.1038/s41467-018-04985-0 C6028641!6028641 !29967419     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29967419 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Commun 2018 ; 9 (1 ): 2570 Nephropedia Template TP {{tp|p=29967419 |t=2018. Human in vivo-generated monocyte-derived dendritic cells and macrophages cross-present antigens through a vacuolar pathway |pdf=|usr=}}{{29967419 }} gab.com Text Human in vivo-generated monocyte-derived dendritic cells and macrophages cross-present antigens through a vacuolar pathway JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=29967419 #FOAvip Presentation of exogenous antigens on MHC-I molecules, termed cross-presentation, is essential for cytotoxic CD8(+) T cell responses. In mice, dendritic cells (DCs) that arise from monocytes (mo-DCs) during inflammation have a key function in these responses by cross-presenting antigens locally in peripheral tissues. Whether human naturally-occurring mo-DCs can cross-present is unknown. Here, we use human mo-DCs and macrophages directly purified from ascites to address this question. Single-cell RNA-seq data show that ascites CD1c(+) DCs contain exclusively monocyte-derived cells. Both ascites mo-DCs and monocyte-derived macrophages cross-present efficiently, but are inefficient for transferring exogenous proteins into their cytosol. Inhibition of cysteine proteases, but not of proteasome, abolishes cross-presentation in these cells. We conclude that human monocyte-derived cells cross-present exclusively using a vacuolar pathway. Finally, only ascites mo-DCs provide co-stimulatory signals to induce effector cytotoxic CD8(+) T cells. Our findings thus provide important insights on how to harness cross-presentation for therapeutic purposes. Twit Text FOAVip Human in vivo-generated monocyte-derived dendritic cells and macrophages cross-present antigens through a vacuolar pathway ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=29967419 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29967419 #FOAvip English Wikipedia <ref>{{Cite pmid|29967419 }}</ref> Human in vivo-generated monocyte-derived dendritic cells and macrophages cross-present antigens through a vacuolar pathway #MMPMID29967419 Tang-Huau TL ; Gueguen P ; Goudot C ; Durand M ; Bohec M ; Baulande S ; Pasquier B ; Amigorena S ; Segura E Nat Commun 2018[Jul]; 9 (1 ): 2570 PMID29967419 show ga Presentation of exogenous antigens on MHC-I molecules, termed cross-presentation, is essential for cytotoxic CD8(+) T cell responses. In mice, dendritic cells (DCs) that arise from monocytes (mo-DCs) during inflammation have a key function in these responses by cross-presenting antigens locally in peripheral tissues. Whether human naturally-occurring mo-DCs can cross-present is unknown. Here, we use human mo-DCs and macrophages directly purified from ascites to address this question. Single-cell RNA-seq data show that ascites CD1c(+) DCs contain exclusively monocyte-derived cells. Both ascites mo-DCs and monocyte-derived macrophages cross-present efficiently, but are inefficient for transferring exogenous proteins into their cytosol. Inhibition of cysteine proteases, but not of proteasome, abolishes cross-presentation in these cells. We conclude that human monocyte-derived cells cross-present exclusively using a vacuolar pathway. Finally, only ascites mo-DCs provide co-stimulatory signals to induce effector cytotoxic CD8(+) T cells. Our findings thus provide important insights on how to harness cross-presentation for therapeutic purposes. |*Antigen Presentation [MESH] |Antigens, CD1/metabolism [MESH] |Ascites/etiology/immunology [MESH] |Blood Buffy Coat/cytology [MESH] |Cell Culture Techniques [MESH] |Cells, Cultured [MESH] |Cross-Priming/*immunology [MESH] |Cytosol/metabolism [MESH] |Dendritic Cells/*immunology/metabolism [MESH] |Female [MESH] |Gene Expression Profiling [MESH] |Glycoproteins/metabolism [MESH] |Healthy Volunteers [MESH] |Humans [MESH] |Macrophages/*immunology/metabolism [MESH] |Male [MESH] |Monocytes/*immunology/metabolism [MESH] |Ovarian Neoplasms/complications/immunology [MESH] |Palatine Tonsil/cytology [MESH] |Sequence Analysis, RNA [MESH] |Single-Cell Analysis [MESH] |T-Lymphocytes, Cytotoxic/immunology/metabolism [MESH]Human in vivo-generated monocyte-derived dendritic cells and macrophag(epmc).pdf DeepDyve Pubget Overpricing