Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29921752
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Viruses
2018 ; 10
(6
): ä Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Phage Genetic Engineering Using CRISPR?Cas Systems
#MMPMID29921752
Hatoum-Aslan A
Viruses
2018[Jun]; 10
(6
): ä PMID29921752
show ga
Since their discovery over a decade ago, the class of prokaryotic immune systems
known as CRISPR?Cas have afforded a suite of genetic tools that have
revolutionized research in model organisms spanning all domains of life.
CRISPR-mediated tools have also emerged for the natural targets of CRISPR?Cas
immunity, the viruses that specifically infect bacteria, or phages. Despite their
status as the most abundant biological entities on the planet, the majority of
phage genes have unassigned functions. This reality underscores the need for
robust genetic tools to study them. Recent reports have demonstrated that
CRISPR?Cas systems, specifically the three major types (I, II, and III), can be
harnessed to genetically engineer phages that infect diverse hosts. Here, the
mechanisms of each of these systems, specific strategies used, and phage editing
efficacies will be reviewed. Due to the relatively wide distribution of
CRISPR?Cas systems across bacteria and archaea, it is anticipated that these
immune systems will provide generally applicable tools that will advance the
mechanistic understanding of prokaryotic viruses and accelerate the development
of novel technologies based on these ubiquitous organisms.
free
free
free
