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10.3390/v10060308 http://scihub22266oqcxt.onion/10.3390/v10060308 C6024815!6024815!29882792     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Viruses 2018 ; 10 (6): ä Nephropedia Template TP {{tp|p=29882792|t=2018. The E coli Global Regulator DksA Reduces Transcription during T4 Infection |pdf=|usr=}}{{29882792}} gab.com Text The E coli Global Regulator DksA Reduces Transcription during T4 Infection JO: Viruses http://www.kidney.de/pget.php?&tw=1&pmid=29882792 #FOAvip Bacteriophage T4 relies on host RNA polymerase to transcribe three promoter classes: early (Pe, requires no viral factors), middle (Pm, requires early proteins MotA and AsiA), and late (Pl, requires middle proteins gp55, gp33, and gp45). Using primer extension, RNA-seq, RT-qPCR, single bursts, and a semi-automated method to document plaque size, we investigated how deletion of DksA or ppGpp, two E. coli global transcription regulators, affects T4 infection. Both ppGpp0 and ?dksA increase T4 wild type (wt) plaque size. However, ppGpp0 does not significantly alter burst size or latent period, and only modestly affects T4 transcript abundance, while ?dksA increases burst size (2-fold) without affecting latent period and increases the levels of several Pe transcripts at 5 min post-infection. In a T4motAam infection, ?dksA increases plaque size and shortens latent period, and the levels of specific middle RNAs increase due to more transcription from Pe?s that extend into these middle genes. We conclude that DksA lowers T4 early gene expression. Consequently, ?dksA results in a more productive wt infection and ameliorates the poor expression of middle genes in a T4motAam infection. As DksA does not inhibit Pe transcription in vitro, regulation may be indirect or perhaps requires additional factors. Twit Text FOAVip The E coli Global Regulator DksA Reduces Transcription during T4 Infection ????Viruses http://www.kidney.de/pget.php?&tw=1&pmid=29882792 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29882792 #FOAvip English Wikipedia <ref>{{Cite pmid|29882792}}</ref> The E coli Global Regulator DksA Reduces Transcription during T4 Infection #MMPMID29882792 Patterson-West J; James TD; Fernández-Coll L; Iben JR; Moon K; Knipling L; Cashel M; Hinton DM Viruses 2018[Jun]; 10 (6): ä PMID29882792show ga Bacteriophage T4 relies on host RNA polymerase to transcribe three promoter classes: early (Pe, requires no viral factors), middle (Pm, requires early proteins MotA and AsiA), and late (Pl, requires middle proteins gp55, gp33, and gp45). Using primer extension, RNA-seq, RT-qPCR, single bursts, and a semi-automated method to document plaque size, we investigated how deletion of DksA or ppGpp, two E. coli global transcription regulators, affects T4 infection. Both ppGpp0 and ?dksA increase T4 wild type (wt) plaque size. However, ppGpp0 does not significantly alter burst size or latent period, and only modestly affects T4 transcript abundance, while ?dksA increases burst size (2-fold) without affecting latent period and increases the levels of several Pe transcripts at 5 min post-infection. In a T4motAam infection, ?dksA increases plaque size and shortens latent period, and the levels of specific middle RNAs increase due to more transcription from Pe?s that extend into these middle genes. We conclude that DksA lowers T4 early gene expression. Consequently, ?dksA results in a more productive wt infection and ameliorates the poor expression of middle genes in a T4motAam infection. As DksA does not inhibit Pe transcription in vitro, regulation may be indirect or perhaps requires additional factors. äThe E coli Global Regulator DksA Reduces Transcription during T4 Infe(epmc).pdf DeepDyve Pubget Overpricing