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10.3390/v10060319 http://scihub22266oqcxt.onion/10.3390/v10060319 C6024754!6024754 !29891797     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=29891797 &cmd=llinks): Failed to open stream: HTTP request failed! 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The First Isolation and Whole Genome Sequencing of Murray Valley Encephalitis Virus from Cerebrospinal Fluid of a Patient with Encephalitis |pdf=|usr=}}{{29891797 }} gab.com Text The First Isolation and Whole Genome Sequencing of Murray Valley Encephalitis Virus from Cerebrospinal Fluid of a Patient with Encephalitis JO: Viruses http://www.kidney.de/pget.php?&tw=1&pmid=29891797 #FOAvip Murray Valley Encephalitis virus (MVEV) is a mosquito-borne Flavivirus. Clinical presentation is rare but severe, with a case fatality rate of 15?30%. Here we report a case of MVEV from the cerebrospinal fluid (CSF) of a patient in the Northern Territory in Australia. Initial diagnosis was performed using both MVEV-specific real-time, and Pan-Flavivirus conventional, Polymerase Chain Reaction (PCR), with confirmation by Sanger sequencing. Subsequent isolation, the first from CSF, was conducted in Vero cells and the observed cytopathic effect was confirmed by increasing viral titre in the real-time PCR. Isolation allowed for full genome sequencing using the Scriptseq V2 RNASeq library preparation kit. A consensus genome for VIDRL-MVE was generated and phylogenetic analysis identified it as Genotype 2. This is the first reported isolation, and full genome sequencing of MVEV from CSF. It is also the first time Genotype 2 has been identified in humans. As such, this case has significant implications for public health surveillance, epidemiology, and the understanding of MVEV evolution. Twit Text FOAVip The First Isolation and Whole Genome Sequencing of Murray Valley Encephalitis Virus from Cerebrospinal Fluid of a Patient with Encephalitis ????Viruses http://www.kidney.de/pget.php?&tw=1&pmid=29891797 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29891797 #FOAvip English Wikipedia <ref>{{Cite pmid|29891797 }}</ref> The First Isolation and Whole Genome Sequencing of Murray Valley Encephalitis Virus from Cerebrospinal Fluid of a Patient with Encephalitis #MMPMID29891797 Russell JS ; Caly L ; Kostecki R ; McGuinness SL ; Carter G ; Bulach D ; Seemann T ; Stinear TP ; Baird R ; Catton M ; Druce J Viruses 2018[Jun]; 10 (6 ): ä PMID29891797 show ga Murray Valley Encephalitis virus (MVEV) is a mosquito-borne Flavivirus. Clinical presentation is rare but severe, with a case fatality rate of 15?30%. Here we report a case of MVEV from the cerebrospinal fluid (CSF) of a patient in the Northern Territory in Australia. Initial diagnosis was performed using both MVEV-specific real-time, and Pan-Flavivirus conventional, Polymerase Chain Reaction (PCR), with confirmation by Sanger sequencing. Subsequent isolation, the first from CSF, was conducted in Vero cells and the observed cytopathic effect was confirmed by increasing viral titre in the real-time PCR. Isolation allowed for full genome sequencing using the Scriptseq V2 RNASeq library preparation kit. A consensus genome for VIDRL-MVE was generated and phylogenetic analysis identified it as Genotype 2. This is the first reported isolation, and full genome sequencing of MVEV from CSF. It is also the first time Genotype 2 has been identified in humans. As such, this case has significant implications for public health surveillance, epidemiology, and the understanding of MVEV evolution. |*Whole Genome Sequencing [MESH] |Animals [MESH] |Cerebrospinal Fluid/*virology [MESH] |Child [MESH] |Chlorocebus aethiops [MESH] |Encephalitis Virus, Murray Valley/*classification/genetics/*isolation & purification [MESH] |Encephalitis, Arbovirus/*virology [MESH] |Genotype [MESH] |Humans [MESH] |Northern Territory [MESH] |Phylogeny [MESH] |Polymerase Chain Reaction [MESH] |Sequence Homology [MESH] |Vero Cells [MESH]The First Isolation and Whole Genome Sequencing of Murray Valley Encep(epmc).pdf DeepDyve Pubget Overpricing