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2018 ; 10
(6
): ä Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Analysis of 19 Highly Conserved Vibrio cholerae Bacteriophages Isolated from
Environmental and Patient Sources Over a Twelve-Year Period
#MMPMID29857590
Angermeyer A
; Das MM
; Singh DV
; Seed KD
Viruses
2018[Jun]; 10
(6
): ä PMID29857590
show ga
The Vibrio cholerae biotype "El Tor" is responsible for all of the current
epidemic and endemic cholera outbreaks worldwide. These outbreaks are clonal, and
it is hypothesized that they originate from the coastal areas near the Bay of
Bengal, where the lytic bacteriophage ICP1 (International Centre for Diarrhoeal
Disease Research, Bangladesh cholera phage 1) specifically preys upon these
pathogenic outbreak strains. ICP1 has also been the dominant bacteriophage found
in cholera patient stools since 2001. However, little is known about the genomic
differences between the ICP1 strains that have been collected over time. Here, we
elucidate the pan-genome and the phylogeny of the ICP1 strains by aligning,
annotating, and analyzing the genomes of 19 distinct isolates that were collected
between 2001 and 2012. Our results reveal that the ICP1 isolates are highly
conserved and possess a large core-genome as well as a smaller, somewhat flexible
accessory-genome. Despite its overall conservation, ICP1 strains have managed to
acquire a number of unknown genes, as well as a CRISPR-Cas system which is known
to be critical for its ongoing struggle for co-evolutionary dominance over its
host. This study describes a foundation on which to construct future molecular
and bioinformatic studies of these V. cholerae-associated bacteriophages.