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2018 ; 10
(6
): ä Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Pseudomonas PB1-Like Phages: Whole Genomes from Metagenomes Offer Insight into an
Abundant Group of Bacteriophages
#MMPMID29914169
Watkins SC
; Sible E
; Putonti C
Viruses
2018[Jun]; 10
(6
): ä PMID29914169
show ga
Despite the abundance, ubiquity and impact of environmental viruses, their
inherent genomic plasticity and extreme diversity pose significant challenges for
the examination of bacteriophages on Earth. Viral metagenomic studies have
offered insight into broader aspects of phage ecology and repeatedly uncover
genes to which we are currently unable to assign function. A combined effort of
phage isolation and metagenomic survey of Chicago’s nearshore waters of
Lake Michigan revealed the presence of Pbunaviruses, relatives of the Pseudomonas
phage PB1. This prompted our expansive investigation of PB1-like phages. Genomic
signatures of PB1-like phages and Pbunaviruses were identified, permitting the
unambiguous distinction between the presence/absence of these phages in soils,
freshwater and wastewater samples, as well as publicly available viral
metagenomic datasets. This bioinformatic analysis led to the de novo assembly of
nine novel PB1-like phage genomes from a metagenomic survey of samples collected
from Lake Michigan. While this study finds that Pbunaviruses are abundant in
various environments of Northern Illinois, genomic variation also exists to a
considerable extent within individual communities.