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10.3390/biom8020028 http://scihub22266oqcxt.onion/10.3390/biom8020028 C6023084!6023084!29772810     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biomolecules 2018 ; 8 (2): ä Nephropedia Template TP {{tp|p=29772810|t=2018. Determination of the Membrane Environment of CD59 in Living Cells |pdf=|usr=}}{{29772810}} gab.com Text Determination of the Membrane Environment of CD59 in Living Cells JO: Biomolecules http://www.kidney.de/pget.php?&tw=1&pmid=29772810 #FOAvip The organization and dynamics of proteins and lipids in the plasma membrane, and their role in membrane functionality, have been subject of a long-lasting debate. Specifically, it is unclear to what extent membrane proteins are affected by their immediate lipid environment and vice versa. Studies on model membranes and plasma membrane vesicles indicated preferences of proteins for lipid phases characterized by different acyl chain order; however, whether such phases do indeed exist in live cells is still not known. Here, we refine a previously developed micropatterning approach combined with single molecule tracking to quantify the influence of the glycosylphosphatidylinositol-anchored (GPI-anchored) protein CD59 on its molecular environment directly in the live cell plasma membrane. We find that locally enriched and immobilized CD59 presents obstacles to the diffusion of fluorescently labeled lipids with a different phase-partitioning behavior independent of cell cholesterol levels and type of lipid. Our results give no evidence for either specific binding of the lipids to CD59 or the existence of nanoscopic ordered membrane regions associated with CD59. Twit Text FOAVip Determination of the Membrane Environment of CD59 in Living Cells ????Biomolecules http://www.kidney.de/pget.php?&tw=1&pmid=29772810 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29772810 #FOAvip English Wikipedia <ref>{{Cite pmid|29772810}}</ref> Determination of the Membrane Environment of CD59 in Living Cells #MMPMID29772810 Fülöp G; Brameshuber M; Arnold AM; Schütz GJ; Sevcsik E Biomolecules 2018[Jun]; 8 (2): ä PMID29772810show ga The organization and dynamics of proteins and lipids in the plasma membrane, and their role in membrane functionality, have been subject of a long-lasting debate. Specifically, it is unclear to what extent membrane proteins are affected by their immediate lipid environment and vice versa. Studies on model membranes and plasma membrane vesicles indicated preferences of proteins for lipid phases characterized by different acyl chain order; however, whether such phases do indeed exist in live cells is still not known. Here, we refine a previously developed micropatterning approach combined with single molecule tracking to quantify the influence of the glycosylphosphatidylinositol-anchored (GPI-anchored) protein CD59 on its molecular environment directly in the live cell plasma membrane. We find that locally enriched and immobilized CD59 presents obstacles to the diffusion of fluorescently labeled lipids with a different phase-partitioning behavior independent of cell cholesterol levels and type of lipid. Our results give no evidence for either specific binding of the lipids to CD59 or the existence of nanoscopic ordered membrane regions associated with CD59. äDetermination of the Membrane Environment of CD59 in Living Cells (epmc).pdf DeepDyve Pubget Overpricing