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10.3389/fmicb.2018.01327 http://scihub22266oqcxt.onion/10.3389/fmicb.2018.01327 C6021497!6021497!29977231     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Front+Microbiol 2018 ; 9 (ä): ä Nephropedia Template TP {{tp|p=29977231|t=2018. Endoplasmic Reticulum Stress Mediated MDRV p10 8 Protein-Induced Cell Cycle Arrest and Apoptosis Through the PERK/eIF2? Pathway |pdf=|usr=}}{{29977231}} gab.com Text Endoplasmic Reticulum Stress Mediated MDRV p10 8 Protein-Induced Cell Cycle Arrest and Apoptosis Through the PERK/eIF2 Pathway JO: Front Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=29977231 #FOAvip In this study, the mechanism of Muscovy duck reovirus (MDRV) p10.8 protein-induced pathogenesis was investigated, with a focus on endoplasmic reticulum (ER) stress. In chicken embryo fibroblasts cell lines (DF1), pCI-neo-flg-p10.8 protein transfection increased the phosphorylation (p-) levels of PERK and eIF2? as shown by Western blotting analysis and led to the dissociation of BiP from PERK as shown by co-immunoprecipitation (Co-IP) analysis. Results of treatment with both ER stress activator and inhibitor further confirmed that p10.8 protein induced ER stress. Subsequently, using flow cytometry analysis, it was also found that p10.8 protein induced cell cycle arrest during the G0/G1 phase. Furthermore, p10.8 transfection increased the phosphorylation levels of PERK and eIF2?, and reduced the expression levels of CDK2, CDK4, and Cyclin E according to Western blotting analysis. Treatment with ER stress activator and ER stress inhibitor after p10.8 protein transfection in DF1 cells further indicated that p10.8 protein induced ER stress, which resulted in cell cycle arrest. The results of knockdown of either PERK or eIF2? genes further confirmed that p10.8 protein-induced ER stress led to cell cycle arrest through the PERK/eIF2? pathway. Further results showed that p10.8 protein induced ER stress and apoptosis in DF1 cells. The expression levels of p-PERK, p-eIF2?, CHOP, cleaved-Caspase12, and cleaved-Caspase3 were increased by p10.8 protein. Test results of treatment with each of Tunicamycin, TUDCA and knockdown of PERK, and eIF2?, confirmed that p10.8 protein induced ER stress involving apoptosis via the PERK/eIF2? pathway. In conclusion, MDRV p10.8 protein induced ER stress that caused cell cycle arrest and apoptosis through the PERK/eIF2? pathway. Twit Text FOAVip Endoplasmic Reticulum Stress Mediated MDRV p10 8 Protein-Induced Cell Cycle Arrest and Apoptosis Through the PERK/eIF2 Pathway ????Front Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=29977231 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29977231 #FOAvip English Wikipedia <ref>{{Cite pmid|29977231}}</ref> Endoplasmic Reticulum Stress Mediated MDRV p10 8 Protein-Induced Cell Cycle Arrest and Apoptosis Through the PERK/eIF2? Pathway #MMPMID29977231 Wang Q; Yuan X; Chen Y; Zheng Q; Xu L; Wu Y Front Microbiol 2018[]; 9 (ä): ä PMID29977231show ga In this study, the mechanism of Muscovy duck reovirus (MDRV) p10.8 protein-induced pathogenesis was investigated, with a focus on endoplasmic reticulum (ER) stress. In chicken embryo fibroblasts cell lines (DF1), pCI-neo-flg-p10.8 protein transfection increased the phosphorylation (p-) levels of PERK and eIF2? as shown by Western blotting analysis and led to the dissociation of BiP from PERK as shown by co-immunoprecipitation (Co-IP) analysis. Results of treatment with both ER stress activator and inhibitor further confirmed that p10.8 protein induced ER stress. Subsequently, using flow cytometry analysis, it was also found that p10.8 protein induced cell cycle arrest during the G0/G1 phase. Furthermore, p10.8 transfection increased the phosphorylation levels of PERK and eIF2?, and reduced the expression levels of CDK2, CDK4, and Cyclin E according to Western blotting analysis. Treatment with ER stress activator and ER stress inhibitor after p10.8 protein transfection in DF1 cells further indicated that p10.8 protein induced ER stress, which resulted in cell cycle arrest. The results of knockdown of either PERK or eIF2? genes further confirmed that p10.8 protein-induced ER stress led to cell cycle arrest through the PERK/eIF2? pathway. Further results showed that p10.8 protein induced ER stress and apoptosis in DF1 cells. The expression levels of p-PERK, p-eIF2?, CHOP, cleaved-Caspase12, and cleaved-Caspase3 were increased by p10.8 protein. Test results of treatment with each of Tunicamycin, TUDCA and knockdown of PERK, and eIF2?, confirmed that p10.8 protein induced ER stress involving apoptosis via the PERK/eIF2? pathway. In conclusion, MDRV p10.8 protein induced ER stress that caused cell cycle arrest and apoptosis through the PERK/eIF2? pathway. äEndoplasmic Reticulum Stress Mediated MDRV p10 8 Protein-Induced Cell (epmc).pdf DeepDyve Pubget Overpricing