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10.1038/s41598-018-28114-5 http://scihub22266oqcxt.onion/10.1038/s41598-018-28114-5 C6021400!6021400 !29950577     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=29950577 &cmd=llinks): Failed to open stream: HTTP request failed! 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Gp41 dynamically interacts with the TCR in the immune synapse and promotes early T cell activation |pdf=|usr=}}{{29950577 }} gab.com Text Gp41 dynamically interacts with the TCR in the immune synapse and promotes early T cell activation JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=29950577 #FOAvip The HIV-1 glycoprotein gp41 critically mediates CD4(+) T-cell infection by HIV-1 during viral entry, assembly, and release. Although multiple immune-regulatory activities of gp41 have been reported, the underlying mechanisms of these activities remain poorly understood. Here we employed multi-colour single molecule localization microscopy (SMLM) to resolve interactions of gp41 proteins with cellular proteins at the plasma membrane (PM) of fixed and live CD4(+) T-cells with resolution of ~20-30?nm. We observed that gp41 clusters dynamically associated with the T cell antigen receptor (TCR) at the immune synapse upon TCR stimulation. This interaction, confirmed by FRET, depended on the virus clone, was reduced by the gp41 ectodomain in tight contacts, and was completely abrogated by mutation of the gp41 transmembrane domain. Strikingly, gp41 preferentially colocalized with phosphorylated TCRs at the PM of activated T-cells and promoted TCR phosphorylation. Gp41 expression also resulted in enhanced CD69 upregulation, and in massive cell death after 24-48?hrs. Our results shed new light on HIV-1 assembly mechanisms at the PM of host T-cells and its impact on TCR stimulation. Twit Text FOAVip Gp41 dynamically interacts with the TCR in the immune synapse and promotes early T cell activation ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=29950577 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29950577 #FOAvip English Wikipedia <ref>{{Cite pmid|29950577 }}</ref> Gp41 dynamically interacts with the TCR in the immune synapse and promotes early T cell activation #MMPMID29950577 Yakovian O ; Schwarzer R ; Sajman J ; Neve-Oz Y ; Razvag Y ; Herrmann A ; Sherman E Sci Rep 2018[Jun]; 8 (1 ): 9747 PMID29950577 show ga The HIV-1 glycoprotein gp41 critically mediates CD4(+) T-cell infection by HIV-1 during viral entry, assembly, and release. Although multiple immune-regulatory activities of gp41 have been reported, the underlying mechanisms of these activities remain poorly understood. Here we employed multi-colour single molecule localization microscopy (SMLM) to resolve interactions of gp41 proteins with cellular proteins at the plasma membrane (PM) of fixed and live CD4(+) T-cells with resolution of ~20-30?nm. We observed that gp41 clusters dynamically associated with the T cell antigen receptor (TCR) at the immune synapse upon TCR stimulation. This interaction, confirmed by FRET, depended on the virus clone, was reduced by the gp41 ectodomain in tight contacts, and was completely abrogated by mutation of the gp41 transmembrane domain. Strikingly, gp41 preferentially colocalized with phosphorylated TCRs at the PM of activated T-cells and promoted TCR phosphorylation. Gp41 expression also resulted in enhanced CD69 upregulation, and in massive cell death after 24-48?hrs. Our results shed new light on HIV-1 assembly mechanisms at the PM of host T-cells and its impact on TCR stimulation. |Cell Line [MESH] |HIV Envelope Protein gp41/genetics/*metabolism [MESH] |Humans [MESH] |Lymphocyte Activation/genetics/*physiology [MESH] |Mutation/genetics [MESH] |Receptors, Antigen, T-Cell/genetics/*metabolism [MESH] |Software [MESH] |Synapses/immunology/*metabolism [MESH]Gp41 dynamically interacts with the TCR in the immune synapse and prom(epmc).pdf DeepDyve Pubget Overpricing