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10.1186/s12917-018-1523-z http://scihub22266oqcxt.onion/10.1186/s12917-018-1523-z C6020318!6020318 !29945605     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=29945605 &cmd=llinks): Failed to open stream: HTTP request failed! 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A novel approach to identifying and quantifying neutrophil extracellular trap formation in septic dogs using immunofluorescence microscopy |pdf=|usr=}}{{29945605 }} gab.com Text A novel approach to identifying and quantifying neutrophil extracellular trap formation in septic dogs using immunofluorescence microscopy JO: BMC Vet Res http://www.kidney.de/pget.php?&tw=1&pmid=29945605 #FOAvip BACKGROUND: Canine neutrophils release neutrophil extracellular traps (NETs) in response to lipopolysaccharide but NETs from clinical septic dogs had not been identified. The primary aim is to describe the methodology of identifying and quantifying neutrophil extracellular traps (NETs) in cytology samples of septic foci in dogs with sepsis using immunofluorescence microscopy. Cytology samples including endotracheal tracheal wash (ETW), bronchoalveolar lavage (BAL), abdominal and pleural effusion collected from 5 dogs (3 septic, 2 non-septic) were fixed, permeabilized and stained for myeloperoxidase (MPO), citrullinated histone H3 (citH3) and cell-free DNA (cfDNA). Fluorescence microscopy was used to identify and quantify NETs in 10 random views at 40× magnification. NETs were identified based on co-localization of MPO, citH3 and cfDNA. NETs were quantified as a ratio (number of NETs: number of neutrophils). Neutrophils were identified based on cytoplasmic MPO, cellular diameter and nuclear morphology. RESULTS: NETs were identified and quantified in all cytology samples collected from septic dogs. A small number of NETs was documented in one dog with sterile chronic bronchitis. No NETs were found in sterile abdominal effusion collected from one dog with congestive heart failure. CONCLUSIONS: Immunofluorescence microscopy could be a useful tool for the study of NETs in dogs with clinical sepsis. Twit Text FOAVip A novel approach to identifying and quantifying neutrophil extracellular trap formation in septic dogs using immunofluorescence microscopy ????BMC Vet Res http://www.kidney.de/pget.php?&tw=1&pmid=29945605 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29945605 #FOAvip English Wikipedia <ref>{{Cite pmid|29945605 }}</ref> A novel approach to identifying and quantifying neutrophil extracellular trap formation in septic dogs using immunofluorescence microscopy #MMPMID29945605 Li RHL ; Johnson LR ; Kohen C ; Tablin F BMC Vet Res 2018[Jun]; 14 (1 ): 210 PMID29945605 show ga BACKGROUND: Canine neutrophils release neutrophil extracellular traps (NETs) in response to lipopolysaccharide but NETs from clinical septic dogs had not been identified. The primary aim is to describe the methodology of identifying and quantifying neutrophil extracellular traps (NETs) in cytology samples of septic foci in dogs with sepsis using immunofluorescence microscopy. Cytology samples including endotracheal tracheal wash (ETW), bronchoalveolar lavage (BAL), abdominal and pleural effusion collected from 5 dogs (3 septic, 2 non-septic) were fixed, permeabilized and stained for myeloperoxidase (MPO), citrullinated histone H3 (citH3) and cell-free DNA (cfDNA). Fluorescence microscopy was used to identify and quantify NETs in 10 random views at 40× magnification. NETs were identified based on co-localization of MPO, citH3 and cfDNA. NETs were quantified as a ratio (number of NETs: number of neutrophils). Neutrophils were identified based on cytoplasmic MPO, cellular diameter and nuclear morphology. RESULTS: NETs were identified and quantified in all cytology samples collected from septic dogs. A small number of NETs was documented in one dog with sterile chronic bronchitis. No NETs were found in sterile abdominal effusion collected from one dog with congestive heart failure. CONCLUSIONS: Immunofluorescence microscopy could be a useful tool for the study of NETs in dogs with clinical sepsis. |Animals [MESH] |Bronchoalveolar Lavage Fluid/cytology [MESH] |Dog Diseases/*diagnosis [MESH] |Dogs [MESH] |Extracellular Traps/*metabolism [MESH] |Microscopy, Fluorescence/methods/*veterinary [MESH]A novel approach to identifying and quantifying neutrophil extracellul(epmc).pdf DeepDyve Pubget Overpricing