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10.1038/s41598-018-27693-7 http://scihub22266oqcxt.onion/10.1038/s41598-018-27693-7 C6018692!6018692!29915203     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=29915203&cmd=llinks): Failed to open stream: HTTP request failed! 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Aberration correction considering curved sample surface shape for non-contact two-photon excitation microscopy with spatial light modulator |pdf=|usr=}}{{29915203}} gab.com Text Aberration correction considering curved sample surface shape for non-contact two-photon excitation microscopy with spatial light modulator JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=29915203 #FOAvip In this paper, excitation light wavefront modulation is performed considering the curved sample surface shape to demonstrate high-quality deep observation using two-photon excitation microscopy (TPM) with a dry objective lens. A large spherical aberration typically occurs when the refractive index (RI) interface between air and the sample is a plane perpendicular to the optical axis. Moreover, the curved sample surface shape and the RI mismatch cause various aberrations, including spherical ones. Consequently, the fluorescence intensity and resolution of the obtained image are degraded in the deep regions. To improve them, we designed a pre-distortion wavefront for correcting the aberration caused by the curved sample surface shape by using a novel, simple optical path length difference calculation method. The excitation light wavefront is modulated to the pre-distortion wavefront by a spatial light modulator incorporated in the TPM system before passing through the interface, where the RI mismatch occurs. Thus, the excitation light is condensed without aberrations. Blood vessels were thereby observed up to an optical depth of 2,000 ?m in a cleared mouse brain by using a dry objective lens. Twit Text FOAVip Aberration correction considering curved sample surface shape for non-contact two-photon excitation microscopy with spatial light modulator ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=29915203 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29915203 #FOAvip English Wikipedia <ref>{{Cite pmid|29915203}}</ref> Aberration correction considering curved sample surface shape for non-contact two-photon excitation microscopy with spatial light modulator #MMPMID29915203 Matsumoto N; Konno A; Inoue T; Okazaki S Sci Rep 2018[]; 8 (ä): ä PMID29915203show ga In this paper, excitation light wavefront modulation is performed considering the curved sample surface shape to demonstrate high-quality deep observation using two-photon excitation microscopy (TPM) with a dry objective lens. A large spherical aberration typically occurs when the refractive index (RI) interface between air and the sample is a plane perpendicular to the optical axis. Moreover, the curved sample surface shape and the RI mismatch cause various aberrations, including spherical ones. Consequently, the fluorescence intensity and resolution of the obtained image are degraded in the deep regions. To improve them, we designed a pre-distortion wavefront for correcting the aberration caused by the curved sample surface shape by using a novel, simple optical path length difference calculation method. The excitation light wavefront is modulated to the pre-distortion wavefront by a spatial light modulator incorporated in the TPM system before passing through the interface, where the RI mismatch occurs. Thus, the excitation light is condensed without aberrations. Blood vessels were thereby observed up to an optical depth of 2,000 ?m in a cleared mouse brain by using a dry objective lens. äAberration correction considering curved sample surface shape for non-(epmc).pdf DeepDyve Pubget Overpricing