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10.1186/s10020-018-0003-z http://scihub22266oqcxt.onion/10.1186/s10020-018-0003-z C6016866/?report=reader!6016866!30134795     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Med 2018 ; 24 (ä): ä Nephropedia Template TP {{tp|p=30134795|t=2018. IL-6 is associated to IGF-1Ec upregulation and Ec peptide secretion, from prostate tumors |pdf=|usr=}}{{30134795}} gab.com Text IL-6 is associated to IGF-1Ec upregulation and Ec peptide secretion, from prostate tumors JO: Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=30134795 #FOAvip Background: Ec peptide (PEc), resulting from the proteolytic cleavage of the IGF-1Ec isoform, is involved in prostate cancer progression and metastasis, whereas in muscle tissue, it is associated with the mobilization of satellite cells prior to repair. Our aim is to determine the physiological conditions associated to the IGF-1Ec upregulation and PEc secretion in prostate tumors, as well as, the effect of tumor PEc on tumor repair. Methods: IGF-1 (mature and isoforms) expression was examined by qRT-PCR, both in prostate cancer cells co-incubated with cells of the immune response (IR) and in tumors. PEc secretion was determined by Multiple Reaction Monitoring.The effect of PEc, on mesenchymal stem cell (MSC) mobilization and repair, was examined using migration and invasion assays, FISH and immunohistochemistry (IHC). The JAK/STAT signaling pathway leading to the IGF1-Ec expression was examined by western blot analysis. Determination of the expression and localization of IL-6 and IGF-1Ec in prostate tumors was examined by qRT-PCR and by IHC. Results: We documented that IL-6 secreted by IR cells activates the JAK2 and STAT3 pathway through IL-6 receptor in cancer cells, leading to the IGF-1Ec upregulation and PEc secretion, as well as to the IL-6 expression and secretion. The resulting PEc, apart from its oncogenic role, also mobilizes MSCs towards the tumor, thus promoting tumor repair. Conclusions: IL-6 leads to the PEc secretion from prostate cancer cells. Apart from its oncogenic role, PEc is also involved in the mobilization of MSCs resulting in tumor repair. Electronic supplementary material: The online version of this article (10.1186/s10020-018-0003-z) contains supplementary material, which is available to authorized users. Twit Text FOAVip IL-6 is associated to IGF-1Ec upregulation and Ec peptide secretion, from prostate tumors ????Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=30134795 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=30134795 #FOAvip English Wikipedia <ref>{{Cite pmid|30134795}}</ref> IL-6 is associated to IGF-1Ec upregulation and Ec peptide secretion, from prostate tumors #MMPMID30134795 Armakolas A; Dimakakos A; Loukogiannaki C; Armakolas N; Antonopoulos A; Florou C; Tsioli P; Papageorgiou E; Alexandrou TP; Stathaki M; Spinos D; Pektasides D; Patsouris E; Koutsilieris M Mol Med 2018[]; 24 (ä): ä PMID30134795show ga Background: Ec peptide (PEc), resulting from the proteolytic cleavage of the IGF-1Ec isoform, is involved in prostate cancer progression and metastasis, whereas in muscle tissue, it is associated with the mobilization of satellite cells prior to repair. Our aim is to determine the physiological conditions associated to the IGF-1Ec upregulation and PEc secretion in prostate tumors, as well as, the effect of tumor PEc on tumor repair. Methods: IGF-1 (mature and isoforms) expression was examined by qRT-PCR, both in prostate cancer cells co-incubated with cells of the immune response (IR) and in tumors. PEc secretion was determined by Multiple Reaction Monitoring.The effect of PEc, on mesenchymal stem cell (MSC) mobilization and repair, was examined using migration and invasion assays, FISH and immunohistochemistry (IHC). The JAK/STAT signaling pathway leading to the IGF1-Ec expression was examined by western blot analysis. Determination of the expression and localization of IL-6 and IGF-1Ec in prostate tumors was examined by qRT-PCR and by IHC. Results: We documented that IL-6 secreted by IR cells activates the JAK2 and STAT3 pathway through IL-6 receptor in cancer cells, leading to the IGF-1Ec upregulation and PEc secretion, as well as to the IL-6 expression and secretion. The resulting PEc, apart from its oncogenic role, also mobilizes MSCs towards the tumor, thus promoting tumor repair. Conclusions: IL-6 leads to the PEc secretion from prostate cancer cells. Apart from its oncogenic role, PEc is also involved in the mobilization of MSCs resulting in tumor repair. Electronic supplementary material: The online version of this article (10.1186/s10020-018-0003-z) contains supplementary material, which is available to authorized users. äIL-6 is associated to IGF-1Ec upregulation and Ec peptide secretion, f(epmc).pdf DeepDyve Pubget Overpricing