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LCK as a Potential Therapeutic Target for Acute Rejection after Kidney
Transplantation: A Bioinformatics Clue
#MMPMID29977931
Jia L
; Jia R
; Li Y
; Li X
; Jia Q
; Zhang H
J Immunol Res
2018[]; 2018
(?): 6451298
PMID29977931
show ga
OBJECTIVES: We aim to identify the key biomarker of acute rejection (AR) after
kidney transplantation via bioinformatics methods. METHODS: The gene expression
data GSE75693 of 30 samples with stable kidney transplantation recipients and 15
AR samples were downloaded and analyzed by the limma package to identify
differentially expressed genes (DEGs). Then, Gene Ontology (GO) functional
enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway
analysis were done to explore the biological functions and potential important
pathways of DEGs. Finally, protein-protein interactions (PPIs) and literature
mining were applied to construct the cocitation network and to select the hub
protein. RESULTS: A total of 437 upregulated genes and 353 downregulated genes
were selected according to P < 0.01 and |log(2)(fold?change)| > 1.0. DEGs of AR
are mainly located on membranes and impact the activation of receptors in immune
responses. In the PPI network, Src kinase, lymphocyte kinase (LCK), CD3G, B2M,
interferon-?, CD3D, tumor necrosis factor, VAV1, and CD3E in the T cell receptor
signaling pathway were selected as important factors, and LCK was identified as
the hub protein. CONCLUSION: LCK, via acting on T-cell receptor, might be a
potential therapeutic target for AR after kidney transplantation.
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