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10.1111/jcmm.13609 http://scihub22266oqcxt.onion/10.1111/jcmm.13609 C6010852!6010852!29566307     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Cell+Mol+Med 2018 ; 22 (7): 3340-52 Nephropedia Template TP {{tp|p=29566307|t=2018. Enhancing amplification of late?outgrowth endothelial cells by bilobalide |pdf=|usr=}}{{29566307}} gab.com Text Enhancing amplification of late outgrowth endothelial cells by bilobalide JO: J Cell Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=29566307 #FOAvip Transfusion of autologous late?outgrowth endothelial cells (OECs) is a promising treatment for restenosis after revascularization. Preparing cells by in vitro amplification is a key step to implement the therapy. This study aimed to demonstrate that bilobalide, a terpenoid, enhances the OEC amplification. Human?, rabbit? and rat OECs and a mouse femoral artery injury model were used. Expanding OECs used endothelial growth medium?2 as the standard culture medium while exploring the mechanisms used endothelial basal medium?2. Proliferation assay used MTT method and BrdU method. Migration assay used the modified Boyden chamber. Intracellular nitric oxide, superoxide anion, hydroxyl radical/peroxynitrite and H2O2 were quantified with DAF?FM DA, dihydroethidium, hydroxyphenyl fluorescein and a H2O2 assay kit, respectively. Activated ERK1/2 and eNOS were tested with the Western blot. Bilobalide concentration?dependently enhanced OEC number increase in vitro. Transfusion of bilobalide?based human OECs into femoral injured athymia nude mouse reduced the intimal hyperplasia. Bilobalide promoted OEC proliferation and migration and increased the intracellular nitric oxide level. L?NAME, a NOS inhibitor, inhibits but not abolishes OEC proliferation, migration and ERK1/2 activation. Bilobalide concentration?dependently enhanced the eNOS Ser?1177 phosphorylation and Thr?495 dephosphorylation in activated OECs. Bilobalide alleviates the increase in hydroxyl radical/peroxynitrite, superoxide anion and H2O2 in proliferating OECs. In conclusion, nitric oxide plays a partial role in OEC proliferation and migration; bilobalide increases OEC nitric oxide production and decreases nitric oxide depletion, promoting the OEC number increase; Bilobalide?based OECs are active in vivo. The findings may simplify the preparation of OECs, facilitating the implementation of the autologous?OECs?transfusion therapy. Twit Text FOAVip Enhancing amplification of late outgrowth endothelial cells by bilobalide ????J Cell Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=29566307 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29566307 #FOAvip English Wikipedia <ref>{{Cite pmid|29566307}}</ref> Enhancing amplification of late?outgrowth endothelial cells by bilobalide #MMPMID29566307 Liu S; Hou X; Chen L; Hu H; Sun Q; Zhao F; Liu C J Cell Mol Med 2018[Jul]; 22 (7): 3340-52 PMID29566307show ga Transfusion of autologous late?outgrowth endothelial cells (OECs) is a promising treatment for restenosis after revascularization. Preparing cells by in vitro amplification is a key step to implement the therapy. This study aimed to demonstrate that bilobalide, a terpenoid, enhances the OEC amplification. Human?, rabbit? and rat OECs and a mouse femoral artery injury model were used. Expanding OECs used endothelial growth medium?2 as the standard culture medium while exploring the mechanisms used endothelial basal medium?2. Proliferation assay used MTT method and BrdU method. Migration assay used the modified Boyden chamber. Intracellular nitric oxide, superoxide anion, hydroxyl radical/peroxynitrite and H2O2 were quantified with DAF?FM DA, dihydroethidium, hydroxyphenyl fluorescein and a H2O2 assay kit, respectively. Activated ERK1/2 and eNOS were tested with the Western blot. Bilobalide concentration?dependently enhanced OEC number increase in vitro. Transfusion of bilobalide?based human OECs into femoral injured athymia nude mouse reduced the intimal hyperplasia. Bilobalide promoted OEC proliferation and migration and increased the intracellular nitric oxide level. L?NAME, a NOS inhibitor, inhibits but not abolishes OEC proliferation, migration and ERK1/2 activation. Bilobalide concentration?dependently enhanced the eNOS Ser?1177 phosphorylation and Thr?495 dephosphorylation in activated OECs. Bilobalide alleviates the increase in hydroxyl radical/peroxynitrite, superoxide anion and H2O2 in proliferating OECs. In conclusion, nitric oxide plays a partial role in OEC proliferation and migration; bilobalide increases OEC nitric oxide production and decreases nitric oxide depletion, promoting the OEC number increase; Bilobalide?based OECs are active in vivo. The findings may simplify the preparation of OECs, facilitating the implementation of the autologous?OECs?transfusion therapy. äEnhancing amplification of late?outgrowth endothelial cells by bilobal(epmc).pdf DeepDyve Pubget Overpricing