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Purification of Peroxisomes in a Self-Generated Gradient #MMPMID12806134
Graham J
ScientificWorldJournal 2002[]; 2 (ä): 1532-5 PMID12806134show ga
In iodixanol, peroxisomes are the densest organelle in the light mitochondrial fraction and are therefore easily separated from the other components (lysosomes, mitochondria, etc.) in a self-generated gradient. Self-generated gradients make sample handling very easy and are highly reproducible but need to be formed in either a vertical, near-vertical, or small volume high-performance fixed-angle rotor. The resolution of the peroxisomes is far superior than that in sucrose and, unlike in Percoll? there is no contamination from endoplasmic reticulum.