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10.1100/tsw.2002.836

http://scihub22266oqcxt.onion/10.1100/tsw.2002.836
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C6009755!6009755!12806134
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suck abstract from ncbi


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pmid12806134      ScientificWorldJournal 2002 ; 2 (ä): 1532-5
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  • Purification of Peroxisomes in a Self-Generated Gradient #MMPMID12806134
  • Graham J
  • ScientificWorldJournal 2002[]; 2 (ä): 1532-5 PMID12806134show ga
  • In iodixanol, peroxisomes are the densest organelle in the light mitochondrial fraction and are therefore easily separated from the other components (lysosomes, mitochondria, etc.) in a self-generated gradient. Self-generated gradients make sample handling very easy and are highly reproducible but need to be formed in either a vertical, near-vertical, or small volume high-performance fixed-angle rotor. The resolution of the peroxisomes is far superior than that in sucrose and, unlike in Percoll? there is no contamination from endoplasmic reticulum.
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