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Purification of Peroxisomes Using a Density Barrier in a Swinging-Bucket Rotor #MMPMID12805925
Graham J
ScientificWorldJournal 2002[]; 2 (ä): 1400-43 PMID12805925show ga
In iodixanol, peroxisomes are the densest organelle in the light mitochondrial fraction and are therefore easily separated from the other components (lysosomes, mitochondria, etc.) in a preformed isosmotic continuous gradient. Because of the large difference in density between peroxisomes and the next densest organelle (mitochondria), a density barrier is effective. The resolution of the peroxisomes is far superior than that in sucrose and, unlike in Percoll®, there is no contamination from endoplasmic reticulum.