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10.1100/tsw.2002.286

http://scihub22266oqcxt.onion/10.1100/tsw.2002.286
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suck abstract from ncbi


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pmid12805929
      ScientificWorldJournal 2002 ; 2 (ä): 1435-9
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  • Fractionation of Golgi, endoplasmic reticulum, and plasma membrane from cultured cells in a preformed continuous iodixanol gradient #MMPMID12805929
  • Graham JM
  • ScientificWorldJournal 2002[May]; 2 (ä): 1435-9 PMID12805929 show ga
  • A continuous iodixanol gradient within the range 0-30% (w/v) iodixanol can resolve the major membrane compartments of the endoplasmic reticulum, Golgi membranes, and plasma membrane from a postnuclear supernatant prepared from a cultured cell homogenate. The precise density range of the gradient and the centrifugation conditions (100,000-200,000 g for 2-16 h) vary with the type of cell and the requirements of the separation. The strategy is widely used to study the processing of proteins within cells.
  • |Animals [MESH]
  • |Biomarkers/analysis [MESH]
  • |COS Cells/chemistry [MESH]
  • |Cell Fractionation/methods [MESH]
  • |Cell Line [MESH]
  • |Cell Membrane/chemistry/*metabolism [MESH]
  • |Centrifugation, Isopycnic/*methods [MESH]
  • |Chlorocebus aethiops [MESH]
  • |Endoplasmic Reticulum/chemistry/*metabolism [MESH]
  • |Golgi Apparatus/chemistry/*metabolism [MESH]


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