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10.1007/s13361-018-1901-6 http://scihub22266oqcxt.onion/10.1007/s13361-018-1901-6 C6003997!6003997!29532326     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29532326.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Am+Soc+Mass+Spectrom 2018 ; 29 (6): 1099-110 Nephropedia Template TP {{tp|p=29532326|t=2018. Comprehensive Proteoform Characterization of Plasma Complement Component C8??? by Hybrid Mass Spectrometry Approaches |pdf=|usr=}}{{29532326}} gab.com Text Comprehensive Proteoform Characterization of Plasma Complement Component C8 by Hybrid Mass Spectrometry Approaches JO: J Am Soc Mass Spectrom http://www.kidney.de/pget.php?&tw=1&pmid=29532326 #FOAvip The human complement hetero-trimeric C8??? (C8) protein assembly (~?150 kDa) is an important component of the membrane attack complex (MAC). C8 initiates membrane penetration and coordinates MAC pore formation. Here, we charted in detail the structural micro-heterogeneity within C8, purified from human plasma, combining high-resolution native mass spectrometry and (glyco)peptide-centric proteomics. The intact C8 proteoform profile revealed at least ~?20 co-occurring MS signals. Additionally, we employed ion exchange chromatography to separate purified C8 into four distinct fractions. Their native MS analysis revealed even more detailed structural micro-heterogeneity on C8. Subsequent peptide-centric analysis, by proteolytic digestion of C8 and LC-MS/MS, provided site-specific quantitative profiles of different types of C8 glycosylation. Combining all this data provides a detailed specification of co-occurring C8 proteoforms, including experimental evidence on N-glycosylation, C-mannosylation, and O-glycosylation. In addition to the known N-glycosylation sites, two more N-glycosylation sites were detected on C8. Additionally, we elucidated the stoichiometry of all C-mannosylation sites in all the thrombospondin-like (TSP) domains of C8? and C8?. Lastly, our data contain the first experimental evidence of O-linked glycans located on C8?. Albeit low abundant, these O-glycans are the first PTMs ever detected on this subunit. By placing the observed PTMs in structural models of free C8 and C8 embedded in the MAC, it may be speculated that some of the newly identified modifications may play a role in the MAC formation.Graphical Abstract?Electronic supplementary material: The online version of this article (10.1007/s13361-018-1901-6) contains supplementary material, which is available to authorized users. Twit Text FOAVip Comprehensive Proteoform Characterization of Plasma Complement Component C8 by Hybrid Mass Spectrometry Approaches ????J Am Soc Mass Spectrom http://www.kidney.de/pget.php?&tw=1&pmid=29532326 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29532326 #FOAvip English Wikipedia <ref>{{Cite pmid|29532326}}</ref> Comprehensive Proteoform Characterization of Plasma Complement Component C8??? by Hybrid Mass Spectrometry Approaches #MMPMID29532326 Franc V; Zhu J; Heck AJR J Am Soc Mass Spectrom 2018[]; 29 (6): 1099-110 PMID29532326show ga The human complement hetero-trimeric C8??? (C8) protein assembly (~?150 kDa) is an important component of the membrane attack complex (MAC). C8 initiates membrane penetration and coordinates MAC pore formation. Here, we charted in detail the structural micro-heterogeneity within C8, purified from human plasma, combining high-resolution native mass spectrometry and (glyco)peptide-centric proteomics. The intact C8 proteoform profile revealed at least ~?20 co-occurring MS signals. Additionally, we employed ion exchange chromatography to separate purified C8 into four distinct fractions. Their native MS analysis revealed even more detailed structural micro-heterogeneity on C8. Subsequent peptide-centric analysis, by proteolytic digestion of C8 and LC-MS/MS, provided site-specific quantitative profiles of different types of C8 glycosylation. Combining all this data provides a detailed specification of co-occurring C8 proteoforms, including experimental evidence on N-glycosylation, C-mannosylation, and O-glycosylation. In addition to the known N-glycosylation sites, two more N-glycosylation sites were detected on C8. Additionally, we elucidated the stoichiometry of all C-mannosylation sites in all the thrombospondin-like (TSP) domains of C8? and C8?. Lastly, our data contain the first experimental evidence of O-linked glycans located on C8?. Albeit low abundant, these O-glycans are the first PTMs ever detected on this subunit. By placing the observed PTMs in structural models of free C8 and C8 embedded in the MAC, it may be speculated that some of the newly identified modifications may play a role in the MAC formation.Graphical Abstract?Electronic supplementary material: The online version of this article (10.1007/s13361-018-1901-6) contains supplementary material, which is available to authorized users. äComprehensive Proteoform Characterization of Plasma Complement Compone(epmc).pdf DeepDyve Pubget Overpricing