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Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Oncotarget 2018 ; 9 (32): 22862-9 Nephropedia Template TP
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Micromolecular methods for diagnosis and therapeutic strategy: a case study #MMPMID29854320
Elbouchtaoui M; Tengher I; Miquel C; Brugière C; Benbara A; Zelek L; Ziol M; Bouhidel F; Janin A; Bousquet G; Leboeuf C
Oncotarget 2018[Apr]; 9 (32): 22862-9 PMID29854320show ga
An intraductal carcinoma, 55 mm across, was diagnosed on a total mastectomy in a 45-year-old woman. The 2 micro-invasive areas found were too small for reliable immunostainings for estrogen, progesterone, and HER2 receptors. In the sentinel lymph-node, a subcapsular tumor embole of about 50 cancer cells was identified on the extemporaneous cryo-cut section, but not on further sections after paraffin-embedding of the sample.Considering this tumor metastatic potential, we decided to assess HER2 status on the metastatic embole using pathological and molecular micro-methods. We laser-microdissected the tumor cells, extracted their DNA, and performed droplet-digital-PCR (ddPCR) for HER2 gene copy number variation. The HER2/RNaseP allele ratio was 5.2 in the laser-microdissected tumor cells, similar to the 5.3 ratio in the HER2-overexpressing breast cancer cell line BT-474.We thus optimized the adjuvant treatment of our patient and she received a trastuzumab-based adjuvant chemotherapy.