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10.2147/OTT.S159026 http://scihub22266oqcxt.onion/10.2147/OTT.S159026 C5962258!5962258!29844685     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Onco+Targets+Ther 2018 ; 11 (ä): 2875-90 Nephropedia Template TP {{tp|p=29844685|t=2018. RIP1 regulates TNF-?-mediated lymphangiogenesis and lymphatic metastasis in gallbladder cancer by modulating the NF-?B-VEGF-C pathway |pdf=|usr=}}{{29844685}} gab.com Text RIP1 regulates TNF- -mediated lymphangiogenesis and lymphatic metastasis in gallbladder cancer by modulating the NF- B-VEGF-C pathway JO: Onco Targets Ther http://www.kidney.de/pget.php?&tw=1&pmid=29844685 #FOAvip Background: Tumor necrosis factor alpha (TNF-?) enhances lymphangiogenesis in gallbladder carcinoma (GBC) via activation of nuclear factor (NF-?B)-dependent vascular endothelial growth factor-C (VEGF-C). Receptor-interacting protein 1 (RIP1) is a multifunctional protein in the TNF-? signaling pathway and is highly expressed in GBC. However, whether RIP1 participates in the signaling pathway of TNF-?-mediated VEGF-C expression that enhances lymphangiogenesis in GBC remains unclear. Methods: The RIP1 protein levels in the GBC-SD and NOZ cells upon stimulation with increasing concentrations of TNF-? as indicated was examined using Western blot. Lentiviral RIP1 shRNA and siI?B? were constructed and transduced respectively them into NOZ and GBC-SD cells, and then PcDNA3.1-RIP1 vectors was transduced into siRIP1 cell lines to reverse RIP1 expression. The protein expression of RIP1, inhibitor of NF-?B alpha (I?B?), p-I?B?, TAK1, NF-?B essential modulator were examined through immunoblotting or immunoprecipitation. Moreover, VEGF-C mRNA levels were measured by quantitative real-time polymerase chain reaction, VEGF-C protein levels were measured by immunoblotting and enzyme-linked immunosorbent assay, and VEGF-C promoter and NF-?B activities were quantified using a dual luciferase reporter assay. The association of NF-?B with the VEGF-C promoter was analysed by chromatin immunoprecipitation assay. A three-dimensional coculture method and orthotopic transplantation nude mice model were used to evaluate lymphatic tube-forming and metastasis ability in GBC cells. The expression of RIP1 protein, TNF-? protein and lymphatic vessels in human GBC tissues was examined by immunohistochemistry, and the dependence between RIP1 protein with TNF-? protein and lymphatic vessel density was analysed. Results: TNF-? dose- and time-dependently increased RIP1 protein expression in the GBC-SD and NOZ cells of GBC, and the strongest effect was observed with a concentration of 50 ng/ml. RIP1 is fundamental for TNF-?-mediated NF-?B activation in GBC cells and can regulate TNF-?-mediated VEGF-C expression at the protein and transcriptional levels through the NF-?B pathway. RIP1 can regulate TNF-?-mediated lymphatic tube formation and metastasis in GBC cells both in vitro and vivo. The average optical density of RIP1 was linearly related to that of TNF-? protein and the lymphatic vessel density in GBC tissues. Conclusion: We conclude that RIP1 regulates TNF-?-mediated lymphangiogenesis and lymph node metastasis in GBC by modulating the NF-?B-VEGF-C pathway. Twit Text FOAVip RIP1 regulates TNF- -mediated lymphangiogenesis and lymphatic metastasis in gallbladder cancer by modulating the NF- B-VEGF-C pathway ????Onco Targets Ther http://www.kidney.de/pget.php?&tw=1&pmid=29844685 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29844685 #FOAvip English Wikipedia <ref>{{Cite pmid|29844685}}</ref> RIP1 regulates TNF-?-mediated lymphangiogenesis and lymphatic metastasis in gallbladder cancer by modulating the NF-?B-VEGF-C pathway #MMPMID29844685 Li CZ; Jiang XJ; Lin B; Hong HJ; Zhu SY; Jiang L; Wang XQ; Tang NH; She FF; Chen YL Onco Targets Ther 2018[]; 11 (ä): 2875-90 PMID29844685show ga Background: Tumor necrosis factor alpha (TNF-?) enhances lymphangiogenesis in gallbladder carcinoma (GBC) via activation of nuclear factor (NF-?B)-dependent vascular endothelial growth factor-C (VEGF-C). Receptor-interacting protein 1 (RIP1) is a multifunctional protein in the TNF-? signaling pathway and is highly expressed in GBC. However, whether RIP1 participates in the signaling pathway of TNF-?-mediated VEGF-C expression that enhances lymphangiogenesis in GBC remains unclear. Methods: The RIP1 protein levels in the GBC-SD and NOZ cells upon stimulation with increasing concentrations of TNF-? as indicated was examined using Western blot. Lentiviral RIP1 shRNA and siI?B? were constructed and transduced respectively them into NOZ and GBC-SD cells, and then PcDNA3.1-RIP1 vectors was transduced into siRIP1 cell lines to reverse RIP1 expression. The protein expression of RIP1, inhibitor of NF-?B alpha (I?B?), p-I?B?, TAK1, NF-?B essential modulator were examined through immunoblotting or immunoprecipitation. Moreover, VEGF-C mRNA levels were measured by quantitative real-time polymerase chain reaction, VEGF-C protein levels were measured by immunoblotting and enzyme-linked immunosorbent assay, and VEGF-C promoter and NF-?B activities were quantified using a dual luciferase reporter assay. The association of NF-?B with the VEGF-C promoter was analysed by chromatin immunoprecipitation assay. A three-dimensional coculture method and orthotopic transplantation nude mice model were used to evaluate lymphatic tube-forming and metastasis ability in GBC cells. The expression of RIP1 protein, TNF-? protein and lymphatic vessels in human GBC tissues was examined by immunohistochemistry, and the dependence between RIP1 protein with TNF-? protein and lymphatic vessel density was analysed. Results: TNF-? dose- and time-dependently increased RIP1 protein expression in the GBC-SD and NOZ cells of GBC, and the strongest effect was observed with a concentration of 50 ng/ml. RIP1 is fundamental for TNF-?-mediated NF-?B activation in GBC cells and can regulate TNF-?-mediated VEGF-C expression at the protein and transcriptional levels through the NF-?B pathway. RIP1 can regulate TNF-?-mediated lymphatic tube formation and metastasis in GBC cells both in vitro and vivo. The average optical density of RIP1 was linearly related to that of TNF-? protein and the lymphatic vessel density in GBC tissues. Conclusion: We conclude that RIP1 regulates TNF-?-mediated lymphangiogenesis and lymph node metastasis in GBC by modulating the NF-?B-VEGF-C pathway. äRIP1 regulates TNF-?-mediated lymphangiogenesis and lymphatic metastas(epmc).pdf DeepDyve Pubget Overpricing