Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1038/s41559-017-0447-5 http://scihub22266oqcxt.onion/10.1038/s41559-017-0447-5 C5957490!5957490!29379187     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Ecol+Evol 2018 ; 2 (3): 537-48 Nephropedia Template TP {{tp|p=29379187|t=2018. Dynamic evolution of regulatory element ensembles in primate CD4+ T-cells |pdf=|usr=}}{{29379187}} gab.com Text Dynamic evolution of regulatory element ensembles in primate CD4+ T-cells JO: Nat Ecol Evol http://www.kidney.de/pget.php?&tw=1&pmid=29379187 #FOAvip How evolutionary changes at enhancers affect the transcription of target genes remains an important open question. Previous comparative studies of gene expression have largely measured the abundance of mRNA, which is affected by post-transcriptional regulatory processes, hence limiting inferences about the mechanisms underlying expression differences. Here we directly measured nascent transcription in primate species, allowing us to separate transcription from post-transcriptional regulation. We used PRO-seq to map RNA polymerases in resting and activated CD4+ T-cells in multiple human, chimpanzee, and rhesus macaque individuals, with rodents as outgroups. We observed general conservation in coding and non-coding transcription, punctuated by numerous differences between species, particularly at distal enhancers and non-coding RNAs. Genes regulated by larger numbers of enhancers are more frequently transcribed at evolutionarily stable levels, despite reduced conservation at individual enhancers. Adaptive nucleotide substitutions are associated with lineage-specific transcription, and at one locus, SGPP2, we predict and experimentally validate that multiple substitutions contribute to human-specific transcription. Collectively, our findings suggest a pervasive role for evolutionary compensation across ensembles of enhancers that jointly regulate target genes. Twit Text FOAVip Dynamic evolution of regulatory element ensembles in primate CD4+ T-cells ????Nat Ecol Evol http://www.kidney.de/pget.php?&tw=1&pmid=29379187 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29379187 #FOAvip English Wikipedia <ref>{{Cite pmid|29379187}}</ref> Dynamic evolution of regulatory element ensembles in primate CD4+ T-cells #MMPMID29379187 Danko CG; Choate LA; Marks BA; Rice EJ; Wang Z; Chu T; Martins AL; Dukler N; Coonrod SA; Tait Wojno ED; Lis JT; Kraus WL; Siepel A Nat Ecol Evol 2018[Mar]; 2 (3): 537-48 PMID29379187show ga How evolutionary changes at enhancers affect the transcription of target genes remains an important open question. Previous comparative studies of gene expression have largely measured the abundance of mRNA, which is affected by post-transcriptional regulatory processes, hence limiting inferences about the mechanisms underlying expression differences. Here we directly measured nascent transcription in primate species, allowing us to separate transcription from post-transcriptional regulation. We used PRO-seq to map RNA polymerases in resting and activated CD4+ T-cells in multiple human, chimpanzee, and rhesus macaque individuals, with rodents as outgroups. We observed general conservation in coding and non-coding transcription, punctuated by numerous differences between species, particularly at distal enhancers and non-coding RNAs. Genes regulated by larger numbers of enhancers are more frequently transcribed at evolutionarily stable levels, despite reduced conservation at individual enhancers. Adaptive nucleotide substitutions are associated with lineage-specific transcription, and at one locus, SGPP2, we predict and experimentally validate that multiple substitutions contribute to human-specific transcription. Collectively, our findings suggest a pervasive role for evolutionary compensation across ensembles of enhancers that jointly regulate target genes. äDynamic evolution of regulatory element ensembles in primate CD4+ T-ce(epmc).pdf DeepDyve Pubget Overpricing