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10.1538/expanim.17-0123 http://scihub22266oqcxt.onion/10.1538/expanim.17-0123 C5955754!5955754!29343656     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Exp+Anim 2018 ; 67 (2): 229-37 Nephropedia Template TP {{tp|p=29343656|t=2018. CRISPR-Cas9-mediated generation of obese and diabetic mouse models |pdf=|usr=}}{{29343656}} gab.com Text CRISPR-Cas9-mediated generation of obese and diabetic mouse models JO: Exp Anim http://www.kidney.de/pget.php?&tw=1&pmid=29343656 #FOAvip Mouse models of obesity (ob/ob) and diabetes (db/db) in which the leptin (Lep) and leptin receptor (Lepr) genes have been mutated, respectively, have contributed to a better understanding of human obesity and type 2 diabetes and to the prevention, diagnosis, and treatment of these metabolic diseases. In this study, we report the first CRISPR-Cas9-induced Lep and Lepr knockout (KO) mouse models by co-microinjection of Cas9 mRNA and sgRNAs that specifically targeted Lep or Lepr in C57BL/6J embryos. Our newly established Lep and Lepr KO mouse models showed phenotypic disorders nearly identical to those found in ob/ob and db/db mice, such as an increase in body weight, hyperglycemia, and hepatic steatosis. Thus, Cas9-generated Lep and Lepr KO mouse lines will be easier for genotyping, to maintain the lines, and to use for future obesity and diabetes research. Twit Text FOAVip CRISPR-Cas9-mediated generation of obese and diabetic mouse models ????Exp Anim http://www.kidney.de/pget.php?&tw=1&pmid=29343656 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29343656 #FOAvip English Wikipedia <ref>{{Cite pmid|29343656}}</ref> CRISPR-Cas9-mediated generation of obese and diabetic mouse models #MMPMID29343656 Roh Ji; Lee J; Park SU; Kang YS; Lee J; Oh AR; Choi DJ; Cha JY; Lee HW Exp Anim 2018[]; 67 (2): 229-37 PMID29343656show ga Mouse models of obesity (ob/ob) and diabetes (db/db) in which the leptin (Lep) and leptin receptor (Lepr) genes have been mutated, respectively, have contributed to a better understanding of human obesity and type 2 diabetes and to the prevention, diagnosis, and treatment of these metabolic diseases. In this study, we report the first CRISPR-Cas9-induced Lep and Lepr knockout (KO) mouse models by co-microinjection of Cas9 mRNA and sgRNAs that specifically targeted Lep or Lepr in C57BL/6J embryos. Our newly established Lep and Lepr KO mouse models showed phenotypic disorders nearly identical to those found in ob/ob and db/db mice, such as an increase in body weight, hyperglycemia, and hepatic steatosis. Thus, Cas9-generated Lep and Lepr KO mouse lines will be easier for genotyping, to maintain the lines, and to use for future obesity and diabetes research. äCRISPR-Cas9-mediated generation of obese and diabetic mouse models (epmc).pdf DeepDyve Pubget Overpricing