Analysis of the early cellular and humoral responses of Galleria mellonella
larvae to infection by Candida albicans
#MMPMID28872999
Sheehan G
; Kavanagh K
Virulence
2018[Jan]; 9
(1
): 163-172
PMID28872999
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Galleria mellonella larvae were administered an inoculum of Candida albicans and
the response to infection over 24 hours was monitored. The yeast cell density in
infected larvae declined initially but replication commenced six hours
post-infection. The hemocyte density decreased from 5.2 × 10(6)/ml to 2.5 ×
10(6)/ml at 2 hours but increased to 4.2 × 106 at 6 hours and decreased
subsequently. Administration of ? - glucan to larvae also caused a fluctuation in
hemocyte density (5.1 ± 0.22 × 10(6)/ml (0 hour) to 6.25 ± 0.25 × 106/ml (6 hour)
(p < 0.05) to 5 ± 2.7 × 106 (24 hour)) and the population showed an increase in
the density of small, granular cells at 24 hours (p < 0.05). Hemocytes from
larvae inoculated with ? - glucan for 6 or 24 hours showed faster killing of C.
albicans cells (53 ± 4.1% (p < 0.01), 64 ± 3.7%, (p < 0.01), respectively) than
hemocytes from control larvae (24 ± 11%) at 60 min. Proteomic analysis indicated
increased abundance of immune related proteins cecropin-A (5 fold) and
prophenoloxidase-activating proteinase-1 (5 fold) 6 hours post infection but by
24 hours there was elevated abundance of muscle (tropomyosin 2 (141 fold),
calponin (66 fold), troponin I (62 fold)) and proteins indicative of cellular
stress (glutathione-S-transferase-like protein (114 fold)), fungal dissemination
(muscle protein 20-like protein (174 fold)) and tissue breakdown (mitochondrial
cytochrome c (10 fold)). Proteins decreased in abundance at 24 hour included ? -
1,3 - glucan recognition protein precursor (29 fold) and prophenoloxidase subunit
2 (25 fold).
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