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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 J+Radiat+Res
2018 ; 59
(2
): 116-121
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Investigation of the non-thermal effects of exposing cells to 70-300 GHz
irradiation using a widely tunable source
#MMPMID29281029
Yaekashiwa N
; Otsuki S
; Hayashi S
; Kawase K
J Radiat Res
2018[Mar]; 59
(2
): 116-121
PMID29281029
show ga
This study investigated the effects of millimeter wave (MMW) irradiation with a
wide range of frequencies on the proliferation and activity of normal human skin
fibroblast (NB1RBG) and human glioblastoma (A172) cells. Very few studies have
focused on low-power, long-term irradiation of cells with a widely tunable
source. Our research examined non-thermal effects on cells exposed to radiation
at low power with tunable frequencies from 70 GHz to 300 GHz. A widely tunable
MMW source was set within a cell culture incubator. To avoid the effect of heat
generation due to irradiation, the intensity was maintained below 10 ?W and the
device was arranged such that the irradiation came from underneath the cells.
Irradiation was performed by sweeping from 70 GHz to 300 GHz in 1.0 GHz steps.
The MMW source was positioned 100 mm away from the container in which the cells
were cultured. Cells were exposed to MMWs for either 3, 70 or 94 h. Measurements
of cell proliferation were made using the alternating current measurement method.
We found no difference in proliferation between cells exposed to MMWs and
unexposed cells. A colorimetric method using novel tetrazolium compound: MTS
[3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium,
inner salt] was used for cell activity and cytotoxicity assays. We found no
difference in cellular activity or toxicity between MMW-exposed cells and sham
cells. Our study thus found no non-thermal effect as a result of exposure of
cells to 70 GHz to 300 GHz of radiation.