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10.12659/MSM.907222 http://scihub22266oqcxt.onion/10.12659/MSM.907222 C5950730!5950730!29708961     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Med+Sci+Monit 2018 ; 24 (ä): 2675-82 Nephropedia Template TP {{tp|p=29708961|t=2018. Cellular Mechanisms of Hepatoprotection Mediated by M2-Like Macrophages |pdf=|usr=}}{{29708961}} gab.com Text Cellular Mechanisms of Hepatoprotection Mediated by M2-Like Macrophages JO: Med Sci Monit http://www.kidney.de/pget.php?&tw=1&pmid=29708961 #FOAvip Background: Acute liver injury in the setting of hepatic fibrosis is an intriguing and still unsettled issue. We previously have demonstrated the protective effects conferred by M2-like macrophages in the fibrotic liver. In the present work, we further decipher the cellular mechanisms governing this hepatoprotection. Material/Methods: Macrophages were isolated from control mice (M0 macrophages), then polarized into M1 or M2 phenotype using IFN-? or IL-4, respectively. Conditioned media (CM) from M0, M1, and M2 macrophages were harvested and applied to M1 macrophages. Cell apoptosis was evaluated by immunostaining and real-time PCR. Similarly, human monocyte-derived macrophages were isolated and polarized, then M0, M1, and M2 CM were applied to HL-7702 or HepG2 cells followed by apoptosis induction. Cell apoptosis was assessed by flow cytometry. Results: For the mouse conditioned medium experiment, stronger expression of cleaved caspase 3 and higher Bax/Bcl-2 mRNA ratio were found in M1 macrophages pretreated with M2 CM compared to those in M1 macrophages pretreated with M0 or M1 CM. Similarly, exposure of HL-7702 and HepG2 cells to either M0 or M1 CM had no significant effect on cell apoptosis. Nevertheless, the frequency of hepatocyte apoptosis was substantially reduced in HL-7702 (from 32.23±2.99 to 15.37±0.69 for Annexin V+/PI+ staining, p<0.01) and HepG2 cells (from 36.1±7.26 to 15.2±1.2 for Annexin V+/PI+ staining, p<0.01) with M2 CM pretreatment. Conclusions: M2-like macrophages exert their hepatoprotective effect by promoting M1-like macrophage apoptosis but protecting against hepatocyte apoptosis. Twit Text FOAVip Cellular Mechanisms of Hepatoprotection Mediated by M2-Like Macrophages ????Med Sci Monit http://www.kidney.de/pget.php?&tw=1&pmid=29708961 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29708961 #FOAvip English Wikipedia <ref>{{Cite pmid|29708961}}</ref> Cellular Mechanisms of Hepatoprotection Mediated by M2-Like Macrophages #MMPMID29708961 Bai L; Fu L; Li L; Ren F; Zheng Q; Liu S; Han Y; Zheng S; Chen Y; Duan Z Med Sci Monit 2018[]; 24 (ä): 2675-82 PMID29708961show ga Background: Acute liver injury in the setting of hepatic fibrosis is an intriguing and still unsettled issue. We previously have demonstrated the protective effects conferred by M2-like macrophages in the fibrotic liver. In the present work, we further decipher the cellular mechanisms governing this hepatoprotection. Material/Methods: Macrophages were isolated from control mice (M0 macrophages), then polarized into M1 or M2 phenotype using IFN-? or IL-4, respectively. Conditioned media (CM) from M0, M1, and M2 macrophages were harvested and applied to M1 macrophages. Cell apoptosis was evaluated by immunostaining and real-time PCR. Similarly, human monocyte-derived macrophages were isolated and polarized, then M0, M1, and M2 CM were applied to HL-7702 or HepG2 cells followed by apoptosis induction. Cell apoptosis was assessed by flow cytometry. Results: For the mouse conditioned medium experiment, stronger expression of cleaved caspase 3 and higher Bax/Bcl-2 mRNA ratio were found in M1 macrophages pretreated with M2 CM compared to those in M1 macrophages pretreated with M0 or M1 CM. Similarly, exposure of HL-7702 and HepG2 cells to either M0 or M1 CM had no significant effect on cell apoptosis. Nevertheless, the frequency of hepatocyte apoptosis was substantially reduced in HL-7702 (from 32.23±2.99 to 15.37±0.69 for Annexin V+/PI+ staining, p<0.01) and HepG2 cells (from 36.1±7.26 to 15.2±1.2 for Annexin V+/PI+ staining, p<0.01) with M2 CM pretreatment. Conclusions: M2-like macrophages exert their hepatoprotective effect by promoting M1-like macrophage apoptosis but protecting against hepatocyte apoptosis. äCellular Mechanisms of Hepatoprotection Mediated by M2-Like Macrophage(epmc).pdf DeepDyve Pubget Overpricing