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10.1002/1873-3468.12979 http://scihub22266oqcxt.onion/10.1002/1873-3468.12979 C5947577!5947577!29355929     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       FEBS+Lett 2018 ; 592 (4): 559-67 Nephropedia Template TP {{tp|p=29355929|t=2018. Small fluorescent molecules for monitoring autophagic flux |pdf=|usr=}}{{29355929}} gab.com Text Small fluorescent molecules for monitoring autophagic flux JO: FEBS Lett http://www.kidney.de/pget.php?&tw=1&pmid=29355929 #FOAvip We have developed two types of fluorescent probes, DALGreen and DAPGreen, for monitoring autophagy, that exhibit fluorescence upon being incorporated into autophagosomes. DALGreen enhances its fluorescence at acidic pH, which is favorable for monitoring late?phase autophagy, whereas DAPGreen remains fluorescent with almost constant brightness during the autophagic process. With these probes that stain autophagosomes as they are being formed, the real?time change of autophagic phenomena of live cells may be traced, which is an advantage over conventional approaches with small molecules that stain mature autophagosomes. The use of both dyes allows monitoring of the membrane dynamics of autophagy in any type of cell without the need for genetic engineering, and therefore, will be useful as a tool to study autophagic phenomena. Twit Text FOAVip Small fluorescent molecules for monitoring autophagic flux ????FEBS Lett http://www.kidney.de/pget.php?&tw=1&pmid=29355929 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29355929 #FOAvip English Wikipedia <ref>{{Cite pmid|29355929}}</ref> Small fluorescent molecules for monitoring autophagic flux #MMPMID29355929 Iwashita H; Sakurai HT; Nagahora N; Ishiyama M; Shioji K; Sasamoto K; Okuma K; Shimizu S; Ueno Y FEBS Lett 2018[Feb]; 592 (4): 559-67 PMID29355929show ga We have developed two types of fluorescent probes, DALGreen and DAPGreen, for monitoring autophagy, that exhibit fluorescence upon being incorporated into autophagosomes. DALGreen enhances its fluorescence at acidic pH, which is favorable for monitoring late?phase autophagy, whereas DAPGreen remains fluorescent with almost constant brightness during the autophagic process. With these probes that stain autophagosomes as they are being formed, the real?time change of autophagic phenomena of live cells may be traced, which is an advantage over conventional approaches with small molecules that stain mature autophagosomes. The use of both dyes allows monitoring of the membrane dynamics of autophagy in any type of cell without the need for genetic engineering, and therefore, will be useful as a tool to study autophagic phenomena. äSmall fluorescent molecules for monitoring autophagic flux (epmc).pdf DeepDyve Pubget Overpricing