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2018 ; 9
(5
): 2312-2325
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Spatial and temporal identification of cerebral infarctions based on multiphoton
microscopic imaging
#MMPMID29760990
Wang S
; Du H
; Lin B
; Liao C
; Zhu X
; Wang X
; Chen H
; Zhuo S
; Jiang L
; Li L
; Tu H
; Chen J
Biomed Opt Express
2018[May]; 9
(5
): 2312-2325
PMID29760990
show ga
Ischemic stroke is a leading cause of death and permanent disability worldwide.
Middle cerebral artery occlusion (MCAO) of variable duration times could be
anticipated to result in varying degrees of injury that evolve spatially over
time. Therefore, investigations following strokes require information concerning
the spatiotemporal dimensions of the ischemic core as well as of perilesional
areas. In the present study, multiphoton microscopy (MPM) based on two-photon
excited fluorescence (TPEF) and second harmonic generation (SHG) was applied to
image such pathophysiological events. The ischemic time-points for evaluation
were set at 6, 24, 48, and 72 hours after MCAO. Our results demonstrated that MPM
has the ability to not only identify the normal and ischemic brain regions, but
also reveal morphological changes of the cortex and striatum at various times
following permanent MCAO. These findings corresponded well with the hematoxylin
and eosin (H&E) stained tissue images. With the technologic progression of
miniaturized imaging devices, MPM can be developed into an effective diagnostic
and monitoring tool for ischemic stroke.