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10.3389/fimmu.2018.00927 http://scihub22266oqcxt.onion/10.3389/fimmu.2018.00927 C5946161!5946161!29780386     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Front+Immunol 2018 ; 9 (ä): ä Nephropedia Template TP {{tp|p=29780386|t=2018. Automated Processing and Evaluation of Anti-Nuclear Antibody Indirect Immunofluorescence Testing |pdf=|usr=}}{{29780386}} gab.com Text Automated Processing and Evaluation of Anti-Nuclear Antibody Indirect Immunofluorescence Testing JO: Front Immunol http://www.kidney.de/pget.php?&tw=1&pmid=29780386 #FOAvip Indirect immunofluorescence (IIF) is considered by the American College of Rheumatology (ACR) and the international consensus on ANA patterns (ICAP) the gold standard for the screening of anti-nuclear antibodies (ANA). As conventional IIF is labor intensive, time-consuming, subjective, and poorly standardized, there have been ongoing efforts to improve the standardization of reagents and to develop automated platforms for assay incubation, microscopy, and evaluation. In this study, the workflow and performance characteristics of a fully automated ANA IIF system (Sprinter XL, EUROPattern Suite, IFA 40: HEp-20-10 cells) were compared to a manual approach using visual microscopy with a filter device for single-well titration and to technologist reading. The Sprinter/EUROPattern system enabled the processing of large daily workload cohorts in less than 8?h and the reduction of labor hands-on time by more than 4?h. Regarding the discrimination of positive from negative samples, the overall agreement of the EUROPattern software with technologist reading was higher (95.6%) than when compared to the current method (89.4%). Moreover, the software was consistent with technologist reading in 80.6?97.5% of patterns and 71.0?93.8% of titers. In conclusion, the Sprinter/EUROPattern system provides substantial labor savings and good concordance with technologist ANA IIF microscopy, thus increasing standardization, laboratory efficiency, and removing subjectivity. Twit Text FOAVip Automated Processing and Evaluation of Anti-Nuclear Antibody Indirect Immunofluorescence Testing ????Front Immunol http://www.kidney.de/pget.php?&tw=1&pmid=29780386 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29780386 #FOAvip English Wikipedia <ref>{{Cite pmid|29780386}}</ref> Automated Processing and Evaluation of Anti-Nuclear Antibody Indirect Immunofluorescence Testing #MMPMID29780386 Ricchiuti V; Adams J; Hardy DJ; Katayev A; Fleming JK Front Immunol 2018[]; 9 (ä): ä PMID29780386show ga Indirect immunofluorescence (IIF) is considered by the American College of Rheumatology (ACR) and the international consensus on ANA patterns (ICAP) the gold standard for the screening of anti-nuclear antibodies (ANA). As conventional IIF is labor intensive, time-consuming, subjective, and poorly standardized, there have been ongoing efforts to improve the standardization of reagents and to develop automated platforms for assay incubation, microscopy, and evaluation. In this study, the workflow and performance characteristics of a fully automated ANA IIF system (Sprinter XL, EUROPattern Suite, IFA 40: HEp-20-10 cells) were compared to a manual approach using visual microscopy with a filter device for single-well titration and to technologist reading. The Sprinter/EUROPattern system enabled the processing of large daily workload cohorts in less than 8?h and the reduction of labor hands-on time by more than 4?h. Regarding the discrimination of positive from negative samples, the overall agreement of the EUROPattern software with technologist reading was higher (95.6%) than when compared to the current method (89.4%). Moreover, the software was consistent with technologist reading in 80.6?97.5% of patterns and 71.0?93.8% of titers. In conclusion, the Sprinter/EUROPattern system provides substantial labor savings and good concordance with technologist ANA IIF microscopy, thus increasing standardization, laboratory efficiency, and removing subjectivity. äAutomated Processing and Evaluation of Anti-Nuclear Antibody Indirect (epmc).pdf DeepDyve Pubget Overpricing