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10.21037/jtd.2018.04.09 http://scihub22266oqcxt.onion/10.21037/jtd.2018.04.09 C5945686!5945686!29780628     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Thorac+Dis 2018 ; 10 (Suppl 7): S820-9 Nephropedia Template TP {{tp|p=29780628|t=2018. Comprehensive targeted super-deep next generation sequencing enhances differential diagnosis of solitary pulmonary nodules |pdf=|usr=}}{{29780628}} gab.com Text Comprehensive targeted super-deep next generation sequencing enhances differential diagnosis of solitary pulmonary nodules JO: J Thorac Dis http://www.kidney.de/pget.php?&tw=1&pmid=29780628 #FOAvip Background: A non-invasive method to predict the malignancy of surgery-candidate solitary pulmonary nodules (SPN) is urgently needed. Methods: Super-depth next generation sequencing (NGS) of 35 paired tissues and plasma DNA was performed as an attempt to develop an early diagnosis approach. Results: Only ~6% of malignant nodule patients had driver mutations in the circulating tumour DNA (ctDNA) with >10,000-fold sequencing depth, and the concordance of mutation between tDNA and ctDNA was 3.9%. The first innovative whole mutation scored model in this study predicted 33.3% of malignant SPN with 100% specificity. Conclusions: These results showed that lung cancer gene-targeted deep capture sequencing is not efficient enough to achieve ideal sensitivity by simply increasing the sequencing depth of ctDNA from early candidates. The sequencing could not be evaluated hotspot mutations in the early tumour stage. Nevertheless, a larger cohort is required to optimize this model, and more techniques may be incorporated to benefit the SPN high-risk population. Twit Text FOAVip Comprehensive targeted super-deep next generation sequencing enhances differential diagnosis of solitary pulmonary nodules ????J Thorac Dis http://www.kidney.de/pget.php?&tw=1&pmid=29780628 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29780628 #FOAvip English Wikipedia <ref>{{Cite pmid|29780628}}</ref> Comprehensive targeted super-deep next generation sequencing enhances differential diagnosis of solitary pulmonary nodules #MMPMID29780628 Ye M; Li S; Huang W; Wang C; Liu L; Liu J; Liu J; Pan H; Deng Q; Tang H; Jiang L; Huang W; Chen X; Shao D; Peng Z; Wu R; Zhong J; Wang Z; Zhang X; Kristiansen K; Wang J; Yin Y; Mao M; He J; Liang W J Thorac Dis 2018[Apr]; 10 (Suppl 7): S820-9 PMID29780628show ga Background: A non-invasive method to predict the malignancy of surgery-candidate solitary pulmonary nodules (SPN) is urgently needed. Methods: Super-depth next generation sequencing (NGS) of 35 paired tissues and plasma DNA was performed as an attempt to develop an early diagnosis approach. Results: Only ~6% of malignant nodule patients had driver mutations in the circulating tumour DNA (ctDNA) with >10,000-fold sequencing depth, and the concordance of mutation between tDNA and ctDNA was 3.9%. The first innovative whole mutation scored model in this study predicted 33.3% of malignant SPN with 100% specificity. Conclusions: These results showed that lung cancer gene-targeted deep capture sequencing is not efficient enough to achieve ideal sensitivity by simply increasing the sequencing depth of ctDNA from early candidates. The sequencing could not be evaluated hotspot mutations in the early tumour stage. Nevertheless, a larger cohort is required to optimize this model, and more techniques may be incorporated to benefit the SPN high-risk population. äComprehensive targeted super-deep next generation sequencing enhances (epmc).pdf DeepDyve Pubget Overpricing