Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=29738554
&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215
Repeated mild traumatic brain injury can cause acute neurologic impairment
without overt structural damage in juvenile rats
#MMPMID29738554
Meconi A
; Wortman RC
; Wright DK
; Neale KJ
; Clarkson M
; Shultz SR
; Christie BR
PLoS One
2018[]; 13
(5
): e0197187
PMID29738554
show ga
Repeated concussion is becoming increasingly recognized as a serious public
health concern around the world. Moreover, there is a greater awareness amongst
health professionals of the potential for repeated pediatric concussions to
detrimentally alter the structure and function of the developing brain. To better
study this issue, we developed an awake closed head injury (ACHI) model that
enabled repeated concussions to be performed reliably and reproducibly in
juvenile rats. A neurological assessment protocol (NAP) score was generated
immediately after each ACHI to help quantify the cumulative effects of repeated
injury on level of consciousness, and basic motor and reflexive capacity. Here we
show that we can produce a repeated ACHI (4 impacts in two days) in both male and
female juvenile rats without significant mortality or pain. We show that both
single and repeated injuries produce acute neurological deficits resembling
clinical concussion symptoms that can be quantified using the NAP score.
Behavioural analyses indicate repeated ACHI acutely impaired spatial memory in
the Barnes maze, and an interesting sex effect was revealed as memory impairment
correlated moderately with poorer NAP score performance in a subset of females.
These cognitive impairments occurred in the absence of motor impairments on the
Rotarod, or emotional changes in the open field and elevated plus mazes. Cresyl
violet histology and structural magnetic resonance imaging (MRI) indicated that
repeated ACHI did not produce significant structural damage. MRI also confirmed
there was no volumetric loss in the cortex, hippocampus, or corpus callosum of
animals at 1 or 7 days post-ACHI. Together these data indicate that the ACHI
model can provide a reliable, high throughput means to study the effects of
concussions in juvenile rats.
free
free
free
