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10.1016/j.celrep.2018.03.045 http://scihub22266oqcxt.onion/10.1016/j.celrep.2018.03.045 C5939577!5939577!29642015     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Rep 2018 ; 23 (2): 596-607 Nephropedia Template TP {{tp|p=29642015|t=2018. Genome-wide CRISPR/Cas9 Screen Identifies Host Factors Essential for Influenza Virus Replication |pdf=|usr=}}{{29642015}} gab.com Text Genome-wide CRISPR/Cas9 Screen Identifies Host Factors Essential for Influenza Virus Replication JO: Cell Rep http://www.kidney.de/pget.php?&tw=1&pmid=29642015 #FOAvip The emergence of influenza A viruses (IAVs) from zoonotic reservoirs poses a great threat to human health. As seasonal vaccines are ineffective against zoonotic strains, and newly transmitted viruses can quickly acquire drug resistance, there remains a need for host-directed therapeutics against IAVs. Here, we performed a genome-scale CRISPR/Cas9 knockout screen in human lung epithelial cells with a human isolate of an avian H5N1 strain. Several genes involved in sialic acid biosynthesis and related glycosylation pathways were highly enriched post-H5N1 selection, including SLC35A1, a sialic acid transporter essential for IAV receptor expression and thus viral entry. Importantly, we have identified capicua (CIC) as a negative regulator of cell-intrinsic immunity, as loss of CIC resulted in heightened antiviral responses and restricted replication of multiple viruses. Therefore, our study demonstrates that the CRISPR/Cas9 system can be utilized for the discovery of host factors critical for the replication of intracellular pathogens. Twit Text FOAVip Genome-wide CRISPR/Cas9 Screen Identifies Host Factors Essential for Influenza Virus Replication ????Cell Rep http://www.kidney.de/pget.php?&tw=1&pmid=29642015 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29642015 #FOAvip English Wikipedia <ref>{{Cite pmid|29642015}}</ref> Genome-wide CRISPR/Cas9 Screen Identifies Host Factors Essential for Influenza Virus Replication #MMPMID29642015 Han J; Perez JT; Chen C; Li Y; Benitez A; Kandasamy M; Lee Y; Andrade J; tenOever B; Manicassamy B Cell Rep 2018[Apr]; 23 (2): 596-607 PMID29642015show ga The emergence of influenza A viruses (IAVs) from zoonotic reservoirs poses a great threat to human health. As seasonal vaccines are ineffective against zoonotic strains, and newly transmitted viruses can quickly acquire drug resistance, there remains a need for host-directed therapeutics against IAVs. Here, we performed a genome-scale CRISPR/Cas9 knockout screen in human lung epithelial cells with a human isolate of an avian H5N1 strain. Several genes involved in sialic acid biosynthesis and related glycosylation pathways were highly enriched post-H5N1 selection, including SLC35A1, a sialic acid transporter essential for IAV receptor expression and thus viral entry. Importantly, we have identified capicua (CIC) as a negative regulator of cell-intrinsic immunity, as loss of CIC resulted in heightened antiviral responses and restricted replication of multiple viruses. Therefore, our study demonstrates that the CRISPR/Cas9 system can be utilized for the discovery of host factors critical for the replication of intracellular pathogens. äGenome-wide CRISPR/Cas9 Screen Identifies Host Factors Essential for I(epmc).pdf DeepDyve Pubget Overpricing