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10.1038/nmat4994 http://scihub22266oqcxt.onion/10.1038/nmat4994 C5936694!5936694 !28967916     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28967916 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Mater 2017 ; 16 (11 ): 1112-1119 Nephropedia Template TP {{tp|p=28967916 |t=2017. Organoid cystogenesis reveals a critical role of microenvironment in human polycystic kidney disease |pdf=|usr=}}{{28967916 }} gab.com Text Organoid cystogenesis reveals a critical role of microenvironment in human polycystic kidney disease JO: Nat Mater http://www.kidney.de/pget.php?&tw=1&pmid=28967916 #FOAvip Polycystic kidney disease (PKD) is a life-threatening disorder, commonly caused by defects in polycystin-1 (PC1) or polycystin-2 (PC2), in which tubular epithelia form fluid-filled cysts. A major barrier to understanding PKD is the absence of human cellular models that accurately and efficiently recapitulate cystogenesis. Previously, we have generated a genetic model of PKD using human pluripotent stem cells and derived kidney organoids. Here we show that systematic substitution of physical components can dramatically increase or decrease cyst formation, unveiling a critical role for microenvironment in PKD. Removal of adherent cues increases cystogenesis 10-fold, producing cysts phenotypically resembling PKD that expand massively to 1-centimetre diameters. Removal of stroma enables outgrowth of PKD cell lines, which exhibit defects in PC1 expression and collagen compaction. Cyclic adenosine monophosphate (cAMP), when added, induces cysts in both PKD organoids and controls. These biomaterials establish a highly efficient model of PKD cystogenesis that directly implicates the microenvironment at the earliest stages of the disease. Twit Text FOAVip Organoid cystogenesis reveals a critical role of microenvironment in human polycystic kidney disease ????Nat Mater http://www.kidney.de/pget.php?&tw=1&pmid=28967916 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28967916 #FOAvip English Wikipedia <ref>{{Cite pmid|28967916 }}</ref> Organoid cystogenesis reveals a critical role of microenvironment in human polycystic kidney disease #MMPMID28967916 Cruz NM ; Song X ; Czerniecki SM ; Gulieva RE ; Churchill AJ ; Kim YK ; Winston K ; Tran LM ; Diaz MA ; Fu H ; Finn LS ; Pei Y ; Himmelfarb J ; Freedman BS Nat Mater 2017[Nov]; 16 (11 ): 1112-1119 PMID28967916 show ga Polycystic kidney disease (PKD) is a life-threatening disorder, commonly caused by defects in polycystin-1 (PC1) or polycystin-2 (PC2), in which tubular epithelia form fluid-filled cysts. A major barrier to understanding PKD is the absence of human cellular models that accurately and efficiently recapitulate cystogenesis. Previously, we have generated a genetic model of PKD using human pluripotent stem cells and derived kidney organoids. Here we show that systematic substitution of physical components can dramatically increase or decrease cyst formation, unveiling a critical role for microenvironment in PKD. Removal of adherent cues increases cystogenesis 10-fold, producing cysts phenotypically resembling PKD that expand massively to 1-centimetre diameters. Removal of stroma enables outgrowth of PKD cell lines, which exhibit defects in PC1 expression and collagen compaction. Cyclic adenosine monophosphate (cAMP), when added, induces cysts in both PKD organoids and controls. These biomaterials establish a highly efficient model of PKD cystogenesis that directly implicates the microenvironment at the earliest stages of the disease. |*Cellular Microenvironment [MESH] |*Models, Biological [MESH] |Cell Line [MESH] |Cyclic AMP/metabolism [MESH] |Gene Expression Regulation [MESH] |Humans [MESH] |Organoids/*metabolism/pathology [MESH] |Polycystic Kidney Diseases/genetics/*metabolism/pathology [MESH]Organoid cystogenesis reveals a critical role of microenvironment in h(epmc).pdf DeepDyve Pubget Overpricing