Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1126/sciimmunol.aan8664 http://scihub22266oqcxt.onion/10.1126/sciimmunol.aan8664 C5931334!5931334 !29352091     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=29352091 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29352091 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Sci+Immunol 2018 ; 3 (19 ): ä Nephropedia Template TP {{tp|p=29352091 |t=2018. Precursors of human CD4(+) cytotoxic T lymphocytes identified by single-cell transcriptome analysis |pdf=|usr=}}{{29352091 }} gab.com Text Precursors of human CD4(+) cytotoxic T lymphocytes identified by single-cell transcriptome analysis JO: Sci Immunol http://www.kidney.de/pget.php?&tw=1&pmid=29352091 #FOAvip CD4(+) cytotoxic T lymphocytes (CD4-CTLs) have been reported to play a protective role in several viral infections. However, little is known in humans about the biology of CD4-CTL generation, their functional properties, and heterogeneity, especially in relation to other well-described CD4(+) memory T cell subsets. We performed single-cell RNA sequencing in more than 9000 cells to unravel CD4-CTL heterogeneity, transcriptional profile, and clonality in humans. Single-cell differential gene expression analysis revealed a spectrum of known transcripts, including several linked to cytotoxic and costimulatory function that are expressed at higher levels in the T(EMRA) (effector memory T cells expressing CD45RA) subset, which is highly enriched for CD4-CTLs, compared with CD4(+) T cells in the central memory (T(CM)) and effector memory (T(EM)) subsets. Simultaneous T cell antigen receptor (TCR) analysis in single cells and bulk subsets revealed that CD4-T(EMRA) cells show marked clonal expansion compared with T(CM) and T(EM) cells and that most of CD4-T(EMRA) were dengue virus (DENV)-specific in donors with previous DENV infection. The profile of CD4-T(EMRA) was highly heterogeneous across donors, with four distinct clusters identified by the single-cell analysis. We identified distinct clusters of CD4-CTL effector and precursor cells in the T(EMRA) subset; the precursor cells shared TCR clonotypes with CD4-CTL effectors and were distinguished by high expression of the interleukin-7 receptor. Our identification of a CD4-CTL precursor population may allow further investigation of how CD4-CTLs arise in humans and, thus, could provide insights into the mechanisms that may be used to generate durable and effective CD4-CTL immunity. Twit Text FOAVip Precursors of human CD4(+) cytotoxic T lymphocytes identified by single-cell transcriptome analysis ????Sci Immunol http://www.kidney.de/pget.php?&tw=1&pmid=29352091 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29352091 #FOAvip English Wikipedia <ref>{{Cite pmid|29352091 }}</ref> Precursors of human CD4(+) cytotoxic T lymphocytes identified by single-cell transcriptome analysis #MMPMID29352091 Patil VS ; Madrigal A ; Schmiedel BJ ; Clarke J ; O'Rourke P ; de Silva AD ; Harris E ; Peters B ; Seumois G ; Weiskopf D ; Sette A ; Vijayanand P Sci Immunol 2018[Jan]; 3 (19 ): ä PMID29352091 show ga CD4(+) cytotoxic T lymphocytes (CD4-CTLs) have been reported to play a protective role in several viral infections. However, little is known in humans about the biology of CD4-CTL generation, their functional properties, and heterogeneity, especially in relation to other well-described CD4(+) memory T cell subsets. We performed single-cell RNA sequencing in more than 9000 cells to unravel CD4-CTL heterogeneity, transcriptional profile, and clonality in humans. Single-cell differential gene expression analysis revealed a spectrum of known transcripts, including several linked to cytotoxic and costimulatory function that are expressed at higher levels in the T(EMRA) (effector memory T cells expressing CD45RA) subset, which is highly enriched for CD4-CTLs, compared with CD4(+) T cells in the central memory (T(CM)) and effector memory (T(EM)) subsets. Simultaneous T cell antigen receptor (TCR) analysis in single cells and bulk subsets revealed that CD4-T(EMRA) cells show marked clonal expansion compared with T(CM) and T(EM) cells and that most of CD4-T(EMRA) were dengue virus (DENV)-specific in donors with previous DENV infection. The profile of CD4-T(EMRA) was highly heterogeneous across donors, with four distinct clusters identified by the single-cell analysis. We identified distinct clusters of CD4-CTL effector and precursor cells in the T(EMRA) subset; the precursor cells shared TCR clonotypes with CD4-CTL effectors and were distinguished by high expression of the interleukin-7 receptor. Our identification of a CD4-CTL precursor population may allow further investigation of how CD4-CTLs arise in humans and, thus, could provide insights into the mechanisms that may be used to generate durable and effective CD4-CTL immunity. |CD4-Positive T-Lymphocytes/*immunology [MESH] |Gene Expression Profiling/methods [MESH] |Humans [MESH] |Immunologic Memory/immunology [MESH] |Leukocytes, Mononuclear/immunology [MESH] |Lymphocyte Activation/immunology [MESH] |Receptors, Antigen, T-Cell/immunology [MESH] |Single-Cell Analysis/methods [MESH] |T-Lymphocytes, Cytotoxic/*immunology [MESH]Precursors of human CD4(+) cytotoxic T lymphocytes identified by singl(epmc).pdf DeepDyve Pubget Overpricing