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10.1186/s12864-018-4713-y http://scihub22266oqcxt.onion/10.1186/s12864-018-4713-y C5930688!5930688!29720086     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       BMC+Genomics 2018 ; 19 (ä): ä Nephropedia Template TP {{tp|p=29720086|t=2018. CLICK: one-step generation of conditional knockout mice |pdf=|usr=}}{{29720086}} gab.com Text CLICK: one-step generation of conditional knockout mice JO: BMC Genomics http://www.kidney.de/pget.php?&tw=1&pmid=29720086 #FOAvip Background: CRISPR/Cas9 enables the targeting of genes in zygotes; however, efficient approaches to create loxP-flanked (floxed) alleles remain elusive. Results: Here, we show that the electroporation of Cas9, two gRNAs, and long single-stranded DNA (lssDNA) into zygotes, termed CLICK (CRISPR with lssDNA inducing conditional knockout alleles), enables the quick generation of floxed alleles in mice and rats. Conclusions: The high efficiency of CLICK provides homozygous knock-ins in oocytes carrying tissue-specific Cre, which allows the one-step generation of conditional knockouts in founder (F0) mice. Electronic supplementary material: The online version of this article (10.1186/s12864-018-4713-y) contains supplementary material, which is available to authorized users. Twit Text FOAVip CLICK: one-step generation of conditional knockout mice ????BMC Genomics http://www.kidney.de/pget.php?&tw=1&pmid=29720086 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29720086 #FOAvip English Wikipedia <ref>{{Cite pmid|29720086}}</ref> CLICK: one-step generation of conditional knockout mice #MMPMID29720086 Miyasaka Y; Uno Y; Yoshimi K; Kunihiro Y; Yoshimura T; Tanaka T; Ishikubo H; Hiraoka Y; Takemoto N; Tanaka T; Ooguchi Y; Skehel P; Aida T; Takeda J; Mashimo T BMC Genomics 2018[]; 19 (ä): ä PMID29720086show ga Background: CRISPR/Cas9 enables the targeting of genes in zygotes; however, efficient approaches to create loxP-flanked (floxed) alleles remain elusive. Results: Here, we show that the electroporation of Cas9, two gRNAs, and long single-stranded DNA (lssDNA) into zygotes, termed CLICK (CRISPR with lssDNA inducing conditional knockout alleles), enables the quick generation of floxed alleles in mice and rats. Conclusions: The high efficiency of CLICK provides homozygous knock-ins in oocytes carrying tissue-specific Cre, which allows the one-step generation of conditional knockouts in founder (F0) mice. Electronic supplementary material: The online version of this article (10.1186/s12864-018-4713-y) contains supplementary material, which is available to authorized users. äCLICK: one-step generation of conditional knockout mice (epmc).pdf DeepDyve Pubget Overpricing