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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Am+J+Trop+Med+Hyg
2018 ; 98
(2
): 625-632
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
A Combined Syndromic Approach to Examine Viral, Bacterial, and Parasitic Agents
among Febrile Patients: A Pilot Study in Kilombero, Tanzania
#MMPMID29280432
Hercik C
; Cosmas L
; Mogeni OD
; Wamola N
; Kohi W
; Houpt E
; Liu J
; Ochieng C
; Onyango C
; Fields B
; Mfinanga S
; Montgomery JM
Am J Trop Med Hyg
2018[Feb]; 98
(2
): 625-632
PMID29280432
show ga
The use of fever syndromic surveillance in sub-Saharan Africa is an effective
approach to determine the prevalence of both malarial and nonmalarial infectious
agents. We collected both blood and naso/oro-pharyngeal (NP/OP) swabs from
consecutive consenting patients ? 1 year of age, with an axillary temperature ?
37.5°C, and symptom onset of ? 5 days. Specimens were analyzed using both acute
febrile illness (AFI) and respiratory TaqMan array cards (Resp TAC) for
multiagent detection of 56 different bloodstream and respiratory agents. In
addition, we collected epidemiologic data to further characterize our patient
population. We enrolled 205 febrile patients, including 70 children (1 < 15 years
of age; 34%) and 135 adults (? 15 years of age; 66%). AFI TAC and Resp TAC were
performed on 191 whole blood specimens and 115 NP/OP specimens, respectively. We
detected nucleic acid for Plasmodium (57%), Leptospira (2%), and dengue virus
(1%) among blood specimens. In addition, we detected 17 different respiratory
agents, most notably, Haemophilus influenzae (64%), Streptococcus pneumonia
(56%), Moraxella catarrhalis (39%), and respiratory syncytial virus (11%) among
NP/OP specimens. Overall median cycle threshold was measured at 26.5. This study
provides a proof-of-concept for the use of a multiagent diagnostic approach for
exploratory research on febrile illness and underscores the utility of
quantitative molecular diagnostics in complex epidemiologic settings of
sub-Saharan Africa.