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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Jpn+J+Cancer+Res
2001 ; 92
(5
): 506-15
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Overexpression of latent transforming growth factor-beta 1 (TGF-beta 1) binding
protein 1 (LTBP-1) in association with TGF-beta 1 in ovarian carcinoma
#MMPMID11376559
Higashi T
; Sasagawa T
; Inoue M
; Oka R
; Shuangying L
; Saijoh K
Jpn J Cancer Res
2001[May]; 92
(5
): 506-15
PMID11376559
show ga
Using the differential display method, latent transforming growth factor-beta 1
(TGF-beta 1) binding protein 1 (LTBP-1) mRNA was identified as one of the
enriched mRNAs in ovarian carcinoma tissues after isolation of genes responsible
for the development of ovarian cancer. Semi-quantitative reverse transcription
(RT)-PCR analysis showed that expression of LTBP-1 and TGF-beta 1 mRNAs was much
higher in both serous and mucinous adenocarcinomas than in their benign
counterparts, including serous and mucinous cystadenomas and cystadenomas of low
malignant potential (LMPs). Immunohistochemical analysis demonstrated that only
proliferating benign adenoma cells were immunoreactive for both LTBP-1 and
TGF-beta 1 proteins. In contrast, most serous and mucinous adenocarcinoma cells
and their surrounding stroma were intensely immunoreactive for LTBP-1 and
TGF-beta 1. LTBP-1 and TGF-beta 1 proteins, and their complex forms were
identified in ovarian carcinoma cell lines and in their culture media by western
blot analysis, suggesting these products were produced in ovarian carcinoma
cells. RT-PCR analysis demonstrated that LTBP-1L, one of the LTBP-1 transcripts
that has a strong activity in targeting the latent form of TGF-beta 1 to
extracellular matrix (ECM), was predominantly expressed in ovarian carcinomas.
Taken together, the results suggest that upregulation of LTBP-1 in ovarian
carcinoma cells may have an important role in distributing TGF-beta1 in the
stromal tissues surrounding carcinoma cells.
|*Gene Expression
[MESH]
|*Intracellular Signaling Peptides and Proteins
[MESH]