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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Jpn+J+Cancer+Res
1999 ; 90
(2
): 188-93
Nephropedia Template TP
gab.com Text
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English Wikipedia
Detection of apoptotic cells in human colorectal cancer by two different in situ
methods: antibody against single-stranded DNA and terminal deoxynucleotidyl
transferase-mediated dUTP-biotin nick end-labeling (TUNEL) methods
#MMPMID10189889
Watanabe I
; Toyoda M
; Okuda J
; Tenjo T
; Tanaka K
; Yamamoto T
; Kawasaki H
; Sugiyama T
; Kawarada Y
; Tanigawa N
Jpn J Cancer Res
1999[Feb]; 90
(2
): 188-93
PMID10189889
show ga
We comparatively investigated the extent of apoptotic cell loss in human
colorectal cancers evaluated by two methods, namely the conventional terminal
deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate
(dUTP)-biotin nick end-labeling (TUNEL) method and immunohistochemistry for
single-stranded (ss) DNA. The apoptotic index (AI) obtained with the TUNEL method
was higher than that shown by the immunohistochemistry for ssDNA. However, a
significant correlation in AIs evaluated by these methods was found. The AIs
obtained by both methods were significantly higher in the advanced cancers than
in the early cancers. Cellular proliferation activity was assessed in terms of
positivity rate (PR) for expression of proliferating cell nuclear antigen (PCNA).
The PR of advanced cancers was significantly higher than that of early cancers.
The present results indicate that immunohistochemistry for ssDNA is useful (as is
the TUNEL method) for evaluation of apoptotic tumor cells in colorectal
carcinomas. In addition, it was confirmed that there is a remarkable increase of
not only proliferation activity, but also tumor cell apoptosis in the process of
progression of colon cancer from early to advanced stages of the disease.