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10.3892/ijo.2018.4346 http://scihub22266oqcxt.onion/10.3892/ijo.2018.4346 C5919711!5919711 !29620261     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 286.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 286.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29620261 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Int+J+Oncol 2018 ; 52 (6 ): 1815-1826 Nephropedia Template TP {{tp|p=29620261 |t=2018. Upregulation of E?cadherin expression mediated by a novel dsRNA suppresses the growth and metastasis of bladder cancer cells by inhibiting ?-catenin/TCF target genes |pdf=|usr=}}{{29620261 }} gab.com Text Upregulation of E cadherin expression mediated by a novel dsRNA suppresses the growth and metastasis of bladder cancer cells by inhibiting -catenin/TCF target genes JO: Int J Oncol http://www.kidney.de/pget.php?&tw=1&pmid=29620261 #FOAvip Low expression levels of E?cadherin are correlated with poor prognosis in patients with bladder cancer (BCa). A small activating RNA (saRNA) targeting a specific promoter region can activate gene expression. In the present study, two small double-stranded RNAs (dsRNAs) targeting the promoter region of human E?cadherin were designed and synthesized, and the regulatory role of saRNAs in E?cadherin expression was investigated. The results of reverse transcription-quantitative polymerase chain reaction and western blotting demonstrated that transfection of dsEcad?346 into the BCa cell lines T24 and 5637 significantly activated E?cadherin expression. Furthermore, transfection of dsEcad?346 and miR?373 induced cell cycle arrest in G0/G1 phase, promoted apoptosis and significantly inhibited migration and invasion of BCa cells. Results of immunofluorescence and western blotting indicated that ?-catenin was redistributed from the nucleus to the cell membrane following transfection of dsEcad?346 and miR?373. Additionally, the expression of ?-catenin/T-cell factor complex (TCF) target genes (c?MYC, matrix metallopeptidase 2, cyclin D1) was suppressed following transfection of BCa cells with saRNA. Silencing of E?cadherin expression blocked the inhibitory effect of dsEcad?346 and miR?373 on BCa cells. In conclusion, a novel designed dsEcad?346 can activate the expression of E?cadherin in BCa cells. saRNA-mediated activation of E?cadherin expression inhibited the growth and metastasis of BCa cells by promoting the redistribution of ?-catenin from nucleus to cell membrane and inhibiting the ?-catenin/TCF target genes. Twit Text FOAVip Upregulation of E cadherin expression mediated by a novel dsRNA suppresses the growth and metastasis of bladder cancer cells by inhibiting -catenin/TCF target genes ????Int J Oncol http://www.kidney.de/pget.php?&tw=1&pmid=29620261 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29620261 #FOAvip English Wikipedia <ref>{{Cite pmid|29620261 }}</ref> Upregulation of E?cadherin expression mediated by a novel dsRNA suppresses the growth and metastasis of bladder cancer cells by inhibiting ?-catenin/TCF target genes #MMPMID29620261 Li C ; Liu J ; Zhang Q ; Cui K ; Ge Q ; Wang C ; Chen Z Int J Oncol 2018[Jun]; 52 (6 ): 1815-1826 PMID29620261 show ga Low expression levels of E?cadherin are correlated with poor prognosis in patients with bladder cancer (BCa). A small activating RNA (saRNA) targeting a specific promoter region can activate gene expression. In the present study, two small double-stranded RNAs (dsRNAs) targeting the promoter region of human E?cadherin were designed and synthesized, and the regulatory role of saRNAs in E?cadherin expression was investigated. The results of reverse transcription-quantitative polymerase chain reaction and western blotting demonstrated that transfection of dsEcad?346 into the BCa cell lines T24 and 5637 significantly activated E?cadherin expression. Furthermore, transfection of dsEcad?346 and miR?373 induced cell cycle arrest in G0/G1 phase, promoted apoptosis and significantly inhibited migration and invasion of BCa cells. Results of immunofluorescence and western blotting indicated that ?-catenin was redistributed from the nucleus to the cell membrane following transfection of dsEcad?346 and miR?373. Additionally, the expression of ?-catenin/T-cell factor complex (TCF) target genes (c?MYC, matrix metallopeptidase 2, cyclin D1) was suppressed following transfection of BCa cells with saRNA. Silencing of E?cadherin expression blocked the inhibitory effect of dsEcad?346 and miR?373 on BCa cells. In conclusion, a novel designed dsEcad?346 can activate the expression of E?cadherin in BCa cells. saRNA-mediated activation of E?cadherin expression inhibited the growth and metastasis of BCa cells by promoting the redistribution of ?-catenin from nucleus to cell membrane and inhibiting the ?-catenin/TCF target genes. |*Up-Regulation [MESH] |Antigens, CD [MESH] |Cadherins/*genetics/metabolism [MESH] |Cell Cycle Checkpoints [MESH] |Cell Line, Tumor [MESH] |Cell Movement/drug effects [MESH] |Cell Proliferation/drug effects [MESH] |Cell Survival/drug effects [MESH] |Gene Expression Regulation, Neoplastic/drug effects [MESH] |Humans [MESH] |Neoplasm Metastasis [MESH] |RNA, Double-Stranded/*pharmacology [MESH] |Signal Transduction [MESH] |TCF Transcription Factors/*metabolism [MESH] |Urinary Bladder Neoplasms/*genetics/metabolism [MESH]Upregulation of E?cadherin expression mediated by a novel dsRNA suppre(epmc).pdf DeepDyve Pubget Overpricing