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1994 ; 85
(5
): 491-8
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Light and electron microscopic analysis of liver sinusoids during
hepatocarcinogenesis with 2-acetylaminofluorene in rats
#MMPMID8014107
Shoji Y
; Kaneda K
; Wake K
; Mishima Y
Jpn J Cancer Res
1994[May]; 85
(5
): 491-8
PMID8014107
show ga
To clarify the sequential changes and morphological differences of the sinusoidal
structures between hepatocellular carcinoma (HCC) and hepatocellular adenoma
(HA), we examined morphological changes of sinusoidal cells and related
structures such as basement membrane during hepatocarcinogenesis in the rat.
During continuous feeding of carcinogenic diets containing 2-acetylaminofluorene
to rats, HA appeared at the 8th week in the peripheral area and then extended
toward the centrolobular area. The appearance of HCC was recognized at the 27th
week. In the HA lesion, the morphology of sinusoidal cells and related structures
was basically the same as that of normal liver except for a slight thickening of
the basement membrane and a decreased amount of vitamin A-lipid droplets of
stellate cells. In HCC, the fenestrations of endothelial cells disappeared and
the basement membrane became continuous, thick and often multilayered. Stellate
cells contained almost no vitamin A-lipid droplets and were associated with
abundant collagen fibers. Kupffer cells and pit cells were not seen inside the
sinusoid. All these features of the sinusoids in HCC resembled the morphological
characteristics of the capillary. The present study has revealed that HCC
possesses sinusoid structures distinct from those of HA. This suggests that HCC
may not derive directly from HA but may develop newly within the HA.