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1993 ; 84
(11
): 1145-9
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Enhancement of tumorigenicity and invasion capacity of rat mammary adenocarcinoma
cells by epidermal growth factor and transforming growth factor-beta
#MMPMID8276719
Li X
; Nagayasu H
; Hamada J
; Hosokawa M
; Takeichi N
Jpn J Cancer Res
1993[Nov]; 84
(11
): 1145-9
PMID8276719
show ga
We have studied the effects of growth factors and cytokines on the tumorigenicity
and invasion capacity of tumor cells by using regressor and progressor tumor cell
lines (ER-1 and ERpP, respectively) derived from an SHR rat mammary
adenocarcinoma. ER-1 cells regress spontaneously whereas ERpP cells show invasive
growth and high metastasis to lung and other organs in syngeneic SHR rats. When
ER-1 cells were pretreated with either epidermal growth factor (EGF) or
transforming growth factor-beta (TGF-beta) for 24 h in vitro, and
intraperitoneally transplanted into SHR rats, they grew and killed the host,
whereas ER-1 cells pretreated with tumor necrosis factor-alpha did not.
Tumorigenicity and invasion capacity of ERpP cells were also enhanced by
treatment with EGF and TGF-beta. The ER-1 cells pretreated with EGF, once grown
in vivo, had acquired irreversible tumorigenicity and invasion capacity without
requiring further EGF treatment, and the enhanced malignancy was irreversible.
These findings suggest that growth factors play an important role in acquisition
of malignancy of tumor cells.