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1993 ; 84
(11
): 1150-8
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Immunohistochemical detection of hepatocyte growth factor/scatter factor in human
cancerous and inflammatory lesions of various organs
#MMPMID8276720
Yoshinaga Y
; Matsuno Y
; Fujita S
; Nakamura T
; Kikuchi M
; Shimosato Y
; Hirohashi S
Jpn J Cancer Res
1993[Nov]; 84
(11
): 1150-8
PMID8276720
show ga
Hepatocyte growth factor (HGF)/scatter factor (SF) is a multifunctional factor
considered to be potentially involved in tissue regeneration, wound healing,
embryogenesis, angiogenesis and cancer invasion. Here we examined
immunohistochemically the distribution of HGF/SF in human tissues, including
cancerous and inflammatory tissues, using anti-HGF antibody. HGF/SF accumulation
was clearly detected in the extracellular matrix, particularly along the basement
membrane, in cancerous and inflammatory tissues, but only a little was detected
in normal tissues. HGF/SF is well known to have a strong affinity for heparin in
vitro, and from the results of our immunohistochemical assay, we considered that
HGF/SF was bound to heparin or heparan sulfate of the extracellular matrix and
basement membrane. HGF/SF was well localized in cancerous and inflammatory
lesions of human lung, liver and pancreas, and in apparently normal tissues of
kidney, adrenal gland and pancreas obtained at autopsy. In lung, HGF/SF was
localized along the basement membranes of cancer cell nests, in the extracellular
matrix of the cancer cell surface, cancer stroma and tissues invaded by cancer,
and the basement membranes of bronchial epithelium and capillary vessels in
inflammatory stroma. Since HGF/SF makes some cancer cells more invasive in vitro,
the accumulation of HGF/SF in cancerous tissue suggests that the invasiveness of
some cancer cells may be increased by HGF/SF in vivo.