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10.1111/j.1349-7006.1993.tb02818.x http://scihub22266oqcxt.onion/10.1111/j.1349-7006.1993.tb02818.x C5919082!5919082 !8276722     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=8276722 &cmd=llinks): Failed to open stream: HTTP request failed! 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Leukemic cell lysis by activated human macrophages: significance of membrane-associated tumor necrosis factor |pdf=|usr=}}{{8276722 }} gab.com Text Leukemic cell lysis by activated human macrophages: significance of membrane-associated tumor necrosis factor JO: Jpn J Cancer Res http://www.kidney.de/pget.php?&tw=1&pmid=8276722 #FOAvip In this study, we analyzed the mechanism(s) of leukemic cell lysis by human macrophages. Peripheral blood monocyte-derived macrophages were activated with recombinant interferon-gamma and lipopolysaccharide and their lytic activity against two leukemic cell lines (K562 and HL-60 cells) was assessed by an 111In releasing assay. Activated macrophages lysed these leukemic cells, and the lytic activity against leukemic cells was almost completely inhibited by anti-tumor necrosis factor (TNF) antibody. The macrophage-lysate prepared from activated macrophages also exhibited significant lytic activity against leukemic cells; this lytic activity was inhibited by anti-TNF antibody. The leukemic cells that we used for the cytotoxicity assays were resistant to recombinant TNF. The culture supernatant of activated macrophages did not show any lytic activity. These findings suggest that cell-associated TNF plays a role in macrophage-mediated cytotoxicity against leukemic cells. Twit Text FOAVip Leukemic cell lysis by activated human macrophages: significance of membrane-associated tumor necrosis factor ????Jpn J Cancer Res http://www.kidney.de/pget.php?&tw=1&pmid=8276722 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=8276722 #FOAvip English Wikipedia <ref>{{Cite pmid|8276722 }}</ref> Leukemic cell lysis by activated human macrophages: significance of membrane-associated tumor necrosis factor #MMPMID8276722 Nakabo Y ; Harakawa N ; Yamamoto K ; Okuma M ; Uno K ; Sasada M Jpn J Cancer Res 1993[Nov]; 84 (11 ): 1174-80 PMID8276722 show ga In this study, we analyzed the mechanism(s) of leukemic cell lysis by human macrophages. Peripheral blood monocyte-derived macrophages were activated with recombinant interferon-gamma and lipopolysaccharide and their lytic activity against two leukemic cell lines (K562 and HL-60 cells) was assessed by an 111In releasing assay. Activated macrophages lysed these leukemic cells, and the lytic activity against leukemic cells was almost completely inhibited by anti-tumor necrosis factor (TNF) antibody. The macrophage-lysate prepared from activated macrophages also exhibited significant lytic activity against leukemic cells; this lytic activity was inhibited by anti-TNF antibody. The leukemic cells that we used for the cytotoxicity assays were resistant to recombinant TNF. The culture supernatant of activated macrophages did not show any lytic activity. These findings suggest that cell-associated TNF plays a role in macrophage-mediated cytotoxicity against leukemic cells. |*Cytotoxicity, Immunologic [MESH] |Cell Membrane/metabolism [MESH] |In Vitro Techniques [MESH] |Macrophage Activation [MESH] |Macrophages/*immunology/metabolism [MESH] |Nitrites/metabolism [MESH] |Tumor Cells, Cultured/cytology/*immunology [MESH]Leukemic cell lysis by activated human macrophages: significance of (epmc).pdf DeepDyve Pubget Overpricing