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1992 ; 83
(6
): 631-7
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Enhancing effect of pokeweed mitogen on the proliferation and the cytotoxicity of
lymphokine-activated killer cells
#MMPMID1379578
Kimoto Y
; Tanaka T
; Fujiwara A
; Fujita M
; Taguchi T
Jpn J Cancer Res
1992[Jun]; 83
(6
): 631-7
PMID1379578
show ga
In order to obtain more potent lymphokine-activated killer (LAK) cells for use in
adoptive immunotherapy, pokeweed mitogen (PWM) was added to the culture medium
for the initial 24-48 h of culturing. The proliferation rate of PWM-stimulated
LAK cells reached about 1000-fold after 3-week culture. This rate was nearly the
same as that of LAK cells stimulated by 10 ng/ml of OKT3, the mouse anti-CD3
monoclonal antibody. However, the cytotoxicity of PWM-stimulated LAK cells was
significantly more potent than that of OKT3-stimulated LAK cells. Phenotypic
analysis revealed that PWM-stimulated LAK cells were CD3+CD56(+)-dominant while
OKT3-stimulated LAK cells were CD3+CD56(-)-dominant. About half of CD3+CD56+
PWM-stimulated LAK cells was CD8+. These results suggest that more efficient
adoptive immunotherapy is possible by using high-dose PWM-stimulated LAK cells
with more potent cytotoxicity. Interleukin-1 beta and tumor necrosis factor alpha
were significantly increased in the culture media after 24-h incubation with 1
micrograms/ml of PWM. Secretion of interferon-gamma was not enhanced by this
concentration of PWM within 24 h. Therefore, PWM is considered to activate
monocytes or macrophages to produce these cytokines in advance, influencing the
proliferation and the cytotoxicity of LAK cells.
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